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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1995-11-6
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pubmed:databankReference |
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pubmed:abstractText |
The pheromone-responding conjugative bacteriocin plasmid pPD1 (59 kb) of Enterococcus faecalis was mapped physically by using a relational clone approach, and transposon analysis with Tn917 (Emr) or Tn916 (Tcr) facilitated the location of the bacteriocin-related genes in a segment of about 6.7 kb. Tn917 insertions within a 3-kb region resulted in constitutive clumping. The nucleotide sequence of the region that included the insertions giving rise to constitutive clumping was determined. The region of pPD1 spanned about 8 kb and was found to contain a number of open reading frames, some of which were named on the basis of homologies with two other pheromone-responding plasmids, pAD1 and pCF10. The genes were arranged in the sequence repB-repA-traC-traB-traA-ipd-traE-traF- orfY-sea-1 with all but repB and traA oriented in the same (left-to-right) direction. traC and traB corresponded, respectively, to traC and traB of pAD1 and to prgY and prgZ of pCF10.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-112916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-113798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-1312528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-1312529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-1315730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-1384040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-14066464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-14066465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-1459967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-1640831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-1749819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-1938961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-2120541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-2128961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-2152897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-2158976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-2550976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-2842316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-2997130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-3005240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-3038841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-3093466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-3104314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-3107464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-3114322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-6245068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-6257641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-6272080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-6292164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-6303955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-6327637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-6411857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-6412228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-6437872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-6782073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-7860595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-8029329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-8349565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-8349566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-8384618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-8432694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-8462105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7559345-98769
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
177
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5574-81
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7559345-Amino Acid Sequence,
pubmed-meshheading:7559345-Bacteriocins,
pubmed-meshheading:7559345-Base Sequence,
pubmed-meshheading:7559345-Cloning, Molecular,
pubmed-meshheading:7559345-Conjugation, Genetic,
pubmed-meshheading:7559345-Enterococcus faecalis,
pubmed-meshheading:7559345-Genes, Bacterial,
pubmed-meshheading:7559345-Genes, Regulator,
pubmed-meshheading:7559345-Molecular Sequence Data,
pubmed-meshheading:7559345-Mutagenesis, Insertional,
pubmed-meshheading:7559345-Oligopeptides,
pubmed-meshheading:7559345-Pheromones,
pubmed-meshheading:7559345-Plasmids,
pubmed-meshheading:7559345-Restriction Mapping,
pubmed-meshheading:7559345-Sequence Analysis, DNA,
pubmed-meshheading:7559345-Sequence Homology, Amino Acid
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pubmed:year |
1995
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pubmed:articleTitle |
Physical mapping of the conjugative bacteriocin plasmid pPD1 of Enterococcus faecalis and identification of the determinant related to the pheromone response.
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pubmed:affiliation |
Department of Microbiology, Gunma University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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