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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4 Pt 1
|
| pubmed:dateCreated |
1995-11-3
|
| pubmed:abstractText |
Evidence suggests that cellular immunity to hepatitis C virus (HCV) core protein may be important in the pathogenesis of viral infection. Therefore, interferon gamma (IFN-gamma) production by peripheral blood mononuclear cells (PBMC) derived from patients with chronic HCV infection (genotype 1b) was examined. The cellular immune response was evaluated with a recombinant HCV core fusion protein derived from a patient with genotype 1b. To identify the immunodominant epitopes, IFN-gamma production in responders was also assessed with a panel of nine synthetic peptides that covered the entire core region. It was found that mononuclear cells from 24 (52%) of 46 patients with chronic liver disease responded to the core protein; asymptomatic HCV carriers demonstrated a lower response rate (14%, P < .05). More important, individuals who had received IFN-alpha treatment and went into clinical and virological remission had a higher response rate (75%, P < .05) compared with those with ongoing hepatitis whose treatment failed (31%). Of 25 patients whose mononuclear cells responded to HCV core protein, 18 had a significant response to one or more peptides; 12 patients reacted to a peptide mixture containing hydrophilic sequences. The core peptide amino acid sequence 141 to 160 was recognized in 9 patients. Interestingly, 7 of 8 patients bearing HLA DR 4 and w53 haplotypes recognized the peptide sequence 141 to 160. Thus, IFN-gamma production of the mononuclear cell response appeared to be HLA DR restricted, and the responding cells were identified as CD4+ T cells.(ABSTRACT TRUNCATED AT 250 WORDS)
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis C Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Core Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/nucleocapsid protein, Hepatitis C...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0270-9139
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
22
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1057-64
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:7557851-Adult,
pubmed-meshheading:7557851-Aged,
pubmed-meshheading:7557851-Amino Acid Sequence,
pubmed-meshheading:7557851-Chronic Disease,
pubmed-meshheading:7557851-Female,
pubmed-meshheading:7557851-Glutathione Transferase,
pubmed-meshheading:7557851-HLA-DR Antigens,
pubmed-meshheading:7557851-Hepacivirus,
pubmed-meshheading:7557851-Hepatitis C,
pubmed-meshheading:7557851-Hepatitis C Antigens,
pubmed-meshheading:7557851-Humans,
pubmed-meshheading:7557851-Interferon-gamma,
pubmed-meshheading:7557851-Japan,
pubmed-meshheading:7557851-Lymphocytes,
pubmed-meshheading:7557851-Male,
pubmed-meshheading:7557851-Middle Aged,
pubmed-meshheading:7557851-Molecular Sequence Data,
pubmed-meshheading:7557851-Peptide Fragments,
pubmed-meshheading:7557851-Recombinant Fusion Proteins,
pubmed-meshheading:7557851-Viral Core Proteins
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Interferon gamma production by peripheral blood lymphocytes to hepatitis C virus core protein in chronic hepatitis C infection.
|
| pubmed:affiliation |
Third Department of Internal Medicine, Nagoya University School of Medicine, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|