Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1995-11-9
|
| pubmed:abstractText |
Detection of hepatitis C virus viremia (HCV RNA) in serum of hemodialysis (HD) patients is crucial for documenting ongoing infection because the clinical and epidemiological importance of anti-HCV positivity is not clear. HCV viremia was studied in 104 HD patients by reverse transcription polymerase chain reaction (RT PCR) using primers localized in the 5' non-coding region of the viral genome. We used two different methods to detect HCV RNA: a direct PCR amplification of HCV RNA from human serum, and a standard RT PCR procedure (requiring the RNA extraction step). There were 50 (48%) anti-HCV positive patients in this population. Twenty-two (21.1%) out of 104 patients showed HCV RNA in serum by standard RT PCR technique: they belonged to the anti-HCV positive patient group, whereas all anti-HCV negative patients were HCV RNA negative. Prevalence of HCV RNA was more than doubled when standard RT PCR was used compared to direct RT PCR protocol. There was a good association between serum HCV RNA and circulating anti-HCV antibodies, tested by second-generation ELISA and RIBA assays. HCV viremia was not associated with either the presence or the absence of a particular RIBA antibody specificity. AST and ALT levels had no predictive value for HCV viremia, because they were repeatedly normal in the majority of viremic patients (16/22: 73%).(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0301-0430
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
44
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
49-55
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7554533-Cross-Sectional Studies,
pubmed-meshheading:7554533-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:7554533-Female,
pubmed-meshheading:7554533-Hepacivirus,
pubmed-meshheading:7554533-Hepatitis C,
pubmed-meshheading:7554533-Hepatitis C Antibodies,
pubmed-meshheading:7554533-Humans,
pubmed-meshheading:7554533-Immunoblotting,
pubmed-meshheading:7554533-Kidney Failure, Chronic,
pubmed-meshheading:7554533-Male,
pubmed-meshheading:7554533-Middle Aged,
pubmed-meshheading:7554533-Polymerase Chain Reaction,
pubmed-meshheading:7554533-Prevalence,
pubmed-meshheading:7554533-RNA, Viral,
pubmed-meshheading:7554533-Renal Dialysis,
pubmed-meshheading:7554533-Sensitivity and Specificity,
pubmed-meshheading:7554533-Seroepidemiologic Studies,
pubmed-meshheading:7554533-Specimen Handling,
pubmed-meshheading:7554533-Viremia
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Virological characteristics of hepatitis C virus infection in chronic hemodialysis patients: a cross-sectional study.
|
| pubmed:affiliation |
Hospital of Lecco, Department of Hygiene and Preventive Medicine, University of Milan, Italy.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|