Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1995-10-25
|
| pubmed:abstractText |
Three-color automated flow cytometry was carried out on peripheral blood CD4+ and CD8+ T-lymphocytes of 42 HIV-positive patients using tri-color anti-CD4 or anti-CD8, phycoerythrin-anti-CD38, and fluorescein-anti-HLA-DR, mAbs to elucidate further the T-cell activation hypothesis recently proposed to explain CD4+ T-cell abnormalities observed during HIV infection. CD4+ CD38+ T-cells constituted the major part of circulating CD4+ T-cells in HIV-infected patients and their HLA-DR molecule positivity increased as their disease progressed. The level of CD38 and HLA-DR expression on CD4+ T-cells was positively correlated to that of CD8+ T-cells and to the level of beta 2-microglobulin. Next, to determine whether CD38 expression was associated with a selective expansion or deletion of V beta gene-defined subsets, we compared the V beta gene frequencies between CD38+ and CD38- T-cells from HIV-infected CDC stage II patients using 13 mAbs specific to V beta families. While selective expansion of certain V beta families was observed in CD4+ and CD8+ T-cells the T-cell receptor V beta subset distribution was similar among CD38+ and CD38-, CD4+ and CD8+ T-cells, suggesting that CD38+ expression was either independent of an HIV-encoded antigen-driven process or rather indicative of T-cell immaturity. It is proposed that the phenotype of circulating CD4+ and CD8+ T-cells of HIV-infected patients is a feature of two different mechanisms: (i) an in vitro activation state responsible for increased DR expression and selective expansion of V beta gene-defined subsets, and (ii) T-cell immaturity due to an increased turnover of these cells and accounting for increased CD38 expression.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADP-ribosyl Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD38,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Antiviral Agents,
http://linkedlifedata.com/resource/pubmed/chemical/CD38 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/N-Glycosyl Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Zidovudine,
http://linkedlifedata.com/resource/pubmed/chemical/beta 2-Microglobulin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0090-1229
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
77
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
33-41
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7554481-ADP-ribosyl Cyclase,
pubmed-meshheading:7554481-Acquired Immunodeficiency Syndrome,
pubmed-meshheading:7554481-Adult,
pubmed-meshheading:7554481-Antigens, CD,
pubmed-meshheading:7554481-Antigens, CD38,
pubmed-meshheading:7554481-Antigens, Differentiation,
pubmed-meshheading:7554481-Antiviral Agents,
pubmed-meshheading:7554481-CD4 Lymphocyte Count,
pubmed-meshheading:7554481-CD4-CD8 Ratio,
pubmed-meshheading:7554481-CD4-Positive T-Lymphocytes,
pubmed-meshheading:7554481-CD8-Positive T-Lymphocytes,
pubmed-meshheading:7554481-Female,
pubmed-meshheading:7554481-Gene Rearrangement, beta-Chain T-Cell Antigen Receptor,
pubmed-meshheading:7554481-HLA-DR Antigens,
pubmed-meshheading:7554481-Humans,
pubmed-meshheading:7554481-Lymphocyte Activation,
pubmed-meshheading:7554481-Male,
pubmed-meshheading:7554481-Membrane Glycoproteins,
pubmed-meshheading:7554481-Middle Aged,
pubmed-meshheading:7554481-N-Glycosyl Hydrolases,
pubmed-meshheading:7554481-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:7554481-T-Lymphocyte Subsets,
pubmed-meshheading:7554481-Zidovudine,
pubmed-meshheading:7554481-beta 2-Microglobulin
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
During HIV infection, CD4+ CD38+ T-cells are the predominant circulating CD4+ subset whose HLA-DR positivity increases with disease progression and whose V beta repertoire is similar to that of CD4+ CD38- T-cells.
|
| pubmed:affiliation |
Groupe de Recherche en Immunopathologie, Institut Fédératif de Recherche Multidisciplinaire sur les Peptides, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|