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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-11-7
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pubmed:abstractText |
Infection of immune cells with HIV induces dysregulation of cytokines which may play a vital role in HIV pathogenesis. We analysed the expression of T helper type 1 (Th1) (interferon-gamma (IFN-gamma)) and Th2 (IL-4, IL-10) type cytokines in peripheral blood lymphocytes (PBL) from HIV+ patients. The semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) analysis revealed that IFN-gamma mRNA in unstimulated PBL was significantly decreased and IL-10 mRNA was significantly upregulated in patients with < 400 CD4+ T cells/mm3 (n = 30) as compared to patients with > 400 CD4+ T cells/mm3 (n = 6) and normal controls (n = 16). In addition, IL-10 mRNA levels were inversely associated with IFN-gamma expression. Similar results were obtained by measuring IL-10 production in the supernatants of PBL cultured in vitro without stimulation by employing an enzyme immunosorbent assay (ELISA). However, the levels of IL-4 and IFN-gamma produced by unstimulated PBL were undetectable by ELISA. Mitogen stimulation of PBL revealed two groups of HIV+ individuals based on IL-10 production. PBL from one set of individuals produced low levels of IL-10 (low IL-10 producers) whereas the other group produced IL-10 comparable to that of normal controls (IL-10 producers). Production of IL-4 was significantly reduced in HIV+ individuals with < 400 CD4+ T cells/mm3 as compared to the normal controls. However, ability to produce IFN-gamma by mitogen-stimulated total PBL and CD4+ purified cells was not impaired in HIV+ individuals. These results suggest that unstimulated and mitogen-stimulated PBL of HIV+ individuals exhibit dysregulation of Th2 type cytokines which may play a role in HIV immunopathogenesis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-1330578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-1387110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-1400376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-1512298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-1655948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-1705278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-1851798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-1901776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-2124252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-2144967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-2167351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-2182729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-2186752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-2193094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-2430298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-2466937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-2574188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-2783441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-2961571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-3047875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-7392958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-7687627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-7821928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-7821929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-7911340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-7946051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-8023142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-8037735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-8096375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-8096699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-8113410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-8258695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-8325338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-8381152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-8409454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-8419468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-8450057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554396-8519087
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
102
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
31-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7554396-Adult,
pubmed-meshheading:7554396-Base Sequence,
pubmed-meshheading:7554396-HIV Seropositivity,
pubmed-meshheading:7554396-Humans,
pubmed-meshheading:7554396-Interferon-gamma,
pubmed-meshheading:7554396-Interleukin-10,
pubmed-meshheading:7554396-Interleukin-4,
pubmed-meshheading:7554396-Lymphocyte Activation,
pubmed-meshheading:7554396-Molecular Sequence Data,
pubmed-meshheading:7554396-Polymerase Chain Reaction,
pubmed-meshheading:7554396-T-Lymphocytes, Helper-Inducer
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pubmed:year |
1995
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pubmed:articleTitle |
Expression of IL-10, IL-4 and interferon-gamma in unstimulated and mitogen-stimulated peripheral blood lymphocytes from HIV-seropositive patients.
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pubmed:affiliation |
Department of Paediatrics, Children's Hospital of Eastern Ontario, University of Ottawa, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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