| pubmed-article:7554395 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7554395 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
| pubmed-article:7554395 | lifeskim:mentions | umls-concept:C0021311 | lld:lifeskim |
| pubmed-article:7554395 | lifeskim:mentions | umls-concept:C2349001 | lld:lifeskim |
| pubmed-article:7554395 | lifeskim:mentions | umls-concept:C0681850 | lld:lifeskim |
| pubmed-article:7554395 | lifeskim:mentions | umls-concept:C1706203 | lld:lifeskim |
| pubmed-article:7554395 | lifeskim:mentions | umls-concept:C2697811 | lld:lifeskim |
| pubmed-article:7554395 | lifeskim:mentions | umls-concept:C0003261 | lld:lifeskim |
| pubmed-article:7554395 | lifeskim:mentions | umls-concept:C1306673 | lld:lifeskim |
| pubmed-article:7554395 | lifeskim:mentions | umls-concept:C1550501 | lld:lifeskim |
| pubmed-article:7554395 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:7554395 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:7554395 | pubmed:dateCreated | 1995-11-7 | lld:pubmed |
| pubmed-article:7554395 | pubmed:abstractText | We evaluated the in vitro antibody production from peripheral blood mononuclear cells (PBMC) against HIV-1 proteins in infected adults. Fifty-four HIV-1 infected patients (four recent seroconverters, 15 asymptomatics with a CD4 count higher than 500/microliters, 27 asymptomatics with a CD4 count between 200 and 500/microliters and eight symptomatic patients) were tested. PBMC were incubated in the presence or absence of 1% pokeweed mitogen (PWM) at 37 degrees C for 8 days. Western blot assay, p24 antigen ELISA and anti-p24 antibody ELISA were performed on serum and culture supernatants. Spontaneous production of anti-env antibody in culture supernatants was evidenced in all subjects. All the positive supernatants for anti-core antibodies (18/54) were derived from asymptomatic patients. PBMC from recent seroconverters and from symptomatic patients did not produce any anti-core antibody. Antibody production decreased after stimulation with PWM. The concentration of p24 antigen did not significantly increase in p24 positive supernatants following acidification (P = 0.1), suggesting that the inability to detect p24 antibody was not due to the anti-p24 antibody complexed to p24 antigen in culture supernatants. In vitro production of anti-p24 antibodies was significantly more frequent in asymptomatic subjects with high CD4+ cell counts (P = 0.02) and was absent in recent seroconverters. This last finding suggests that during the initial phases of the infection, anti-p24 antibody production may be restricted to cells residing in lymphoid organs. In addition, the lower percentage of anti-core antibody in people with low CD4+ cell counts is not merely a consequence of the binding of the antibody to an increased amount of antigen, but probably reflects an impaired production or a sequestration of producing cells in lymphoid tissue during the late stages of the infection. | lld:pubmed |
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| pubmed-article:7554395 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7554395 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7554395 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7554395 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7554395 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7554395 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:7554395 | pubmed:issn | 0009-9104 | lld:pubmed |
| pubmed-article:7554395 | pubmed:author | pubmed-author:RivaAA | lld:pubmed |
| pubmed-article:7554395 | pubmed:author | pubmed-author:GalliMM | lld:pubmed |
| pubmed-article:7554395 | pubmed:author | pubmed-author:ScapellatoLL | lld:pubmed |
| pubmed-article:7554395 | pubmed:author | pubmed-author:ZehenderGG | lld:pubmed |
| pubmed-article:7554395 | pubmed:author | pubmed-author:CocchiFF | lld:pubmed |
| pubmed-article:7554395 | pubmed:author | pubmed-author:RusconiSS | lld:pubmed |
| pubmed-article:7554395 | pubmed:author | pubmed-author:MeroniLL | lld:pubmed |
| pubmed-article:7554395 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7554395 | pubmed:volume | 102 | lld:pubmed |
| pubmed-article:7554395 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7554395 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7554395 | pubmed:pagination | 26-30 | lld:pubmed |
| pubmed-article:7554395 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:7554395 | pubmed:meshHeading | pubmed-meshheading:7554395-... | lld:pubmed |
| pubmed-article:7554395 | pubmed:meshHeading | pubmed-meshheading:7554395-... | lld:pubmed |
| pubmed-article:7554395 | pubmed:meshHeading | pubmed-meshheading:7554395-... | lld:pubmed |
| pubmed-article:7554395 | pubmed:meshHeading | pubmed-meshheading:7554395-... | lld:pubmed |
| pubmed-article:7554395 | pubmed:meshHeading | pubmed-meshheading:7554395-... | lld:pubmed |
| pubmed-article:7554395 | pubmed:meshHeading | pubmed-meshheading:7554395-... | lld:pubmed |
| pubmed-article:7554395 | pubmed:meshHeading | pubmed-meshheading:7554395-... | lld:pubmed |
| pubmed-article:7554395 | pubmed:meshHeading | pubmed-meshheading:7554395-... | lld:pubmed |
| pubmed-article:7554395 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7554395 | pubmed:articleTitle | In vitro anti-HIV-1 antibody production in subjects in different stages of HIV-1 infection. | lld:pubmed |
| pubmed-article:7554395 | pubmed:affiliation | Clinica delle Malattie Infettive, Università di Milano, Italy. | lld:pubmed |
| pubmed-article:7554395 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7554395 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7554395 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
