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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-11-7
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pubmed:abstractText |
We evaluated the in vitro antibody production from peripheral blood mononuclear cells (PBMC) against HIV-1 proteins in infected adults. Fifty-four HIV-1 infected patients (four recent seroconverters, 15 asymptomatics with a CD4 count higher than 500/microliters, 27 asymptomatics with a CD4 count between 200 and 500/microliters and eight symptomatic patients) were tested. PBMC were incubated in the presence or absence of 1% pokeweed mitogen (PWM) at 37 degrees C for 8 days. Western blot assay, p24 antigen ELISA and anti-p24 antibody ELISA were performed on serum and culture supernatants. Spontaneous production of anti-env antibody in culture supernatants was evidenced in all subjects. All the positive supernatants for anti-core antibodies (18/54) were derived from asymptomatic patients. PBMC from recent seroconverters and from symptomatic patients did not produce any anti-core antibody. Antibody production decreased after stimulation with PWM. The concentration of p24 antigen did not significantly increase in p24 positive supernatants following acidification (P = 0.1), suggesting that the inability to detect p24 antibody was not due to the anti-p24 antibody complexed to p24 antigen in culture supernatants. In vitro production of anti-p24 antibodies was significantly more frequent in asymptomatic subjects with high CD4+ cell counts (P = 0.02) and was absent in recent seroconverters. This last finding suggests that during the initial phases of the infection, anti-p24 antibody production may be restricted to cells residing in lymphoid organs. In addition, the lower percentage of anti-core antibody in people with low CD4+ cell counts is not merely a consequence of the binding of the antibody to an increased amount of antigen, but probably reflects an impaired production or a sequestration of producing cells in lymphoid tissue during the late stages of the infection.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-1358985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-1371925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-1500743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-1503683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-1671648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-1723280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-1737846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-1899349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-1899628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-1909874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-1974765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-2078296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-2113075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-2147550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-2261128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-2567244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-2703528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-2820544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-2974045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-2991618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-3016028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-3016902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-3024007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-3099912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-3126959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-3257430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-3299092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-3479517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-3492262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-3499462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-3955884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-6224088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-7114072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-7851008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7554395-8095146
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
102
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
26-30
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1995
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pubmed:articleTitle |
In vitro anti-HIV-1 antibody production in subjects in different stages of HIV-1 infection.
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pubmed:affiliation |
Clinica delle Malattie Infettive, Università di Milano, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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