Linked Life Data

7553141

Source:http://linkedlifedata.com/resource/pubmed/id/7553141

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1995-10-26
pubmed:abstractText
The sera of patients with chronic infection of Schistosoma japonicum (Sj) were used for screening the cDNA expression library constructed from Sj adult worm, and an antigen-positive clone Sjgt4 which expressed an 148 KD fusion protein was identified. The inserted Sj cDNA fragment was amplified by PCR and subcloned into the vector pGEX with high level of expression. A positive clone SjpG5 which expressed a fusion protein with a molecular weight of 58 KD was obtained. When the fusion protein was used for detection of Sj patients' sera by Western blot, 24 out of 25 sera were positive, a positive rate of 96%.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0376-2491
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
329-32, 381
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
[Isolation and expression of recombinant diagnostic antigen gene of Schistosoma japonicum].
pubmed:affiliation
Department of Parasitology, Nanjing Medical University.
pubmed:publicationType
Journal Article, English Abstract