|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0007765,
umls-concept:C0010453,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0079925,
umls-concept:C0205225,
umls-concept:C0235032,
umls-concept:C0596630,
umls-concept:C1720655,
umls-concept:C1947974,
umls-concept:C2700455
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|
pubmed:issue |
7
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|
pubmed:dateCreated |
1995-11-7
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|
pubmed:abstractText |
Short-term exposure of primary cultures of cerebellar granule cells from neonatal rat brain to high concentrations of glutamate resulted in a significant increase of both immunoreactivity to and mRNA levels of tau protein. Time-course experiments revealed the increases of tau immunoreactivity and mRNA levels to be maximal 2 h after the glutamate pulse. To investigate the relationship between newly synthesized tau protein and glutamate-induced neurotoxicity, neurons were preincubated with a specific tau antisense oligonucleotide. This treatment resulted in (i) inhibition of the glutamate-induced increase of tau immunoreactivity and (ii) a decrease in the sensitivity of the neurons to neurotoxic concentrations of glutamate. These data indicate that induction of the cytoskeleton-associated tau protein participates in the cascade of events promoted by glutamate leading to neurodegeneration.
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pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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|
pubmed:month |
Jul
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pubmed:issn |
0953-816X
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
7
|
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
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pubmed:pagination |
1603-13
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7551187-Animals,
pubmed-meshheading:7551187-Antisense Elements (Genetics),
pubmed-meshheading:7551187-Base Sequence,
pubmed-meshheading:7551187-Blotting, Western,
pubmed-meshheading:7551187-Calcium,
pubmed-meshheading:7551187-Cells, Cultured,
pubmed-meshheading:7551187-Cerebellum,
pubmed-meshheading:7551187-Glutamic Acid,
pubmed-meshheading:7551187-Intracellular Membranes,
pubmed-meshheading:7551187-Molecular Sequence Data,
pubmed-meshheading:7551187-Neurotoxins,
pubmed-meshheading:7551187-Oligonucleotide Probes,
pubmed-meshheading:7551187-Oligonucleotides, Antisense,
pubmed-meshheading:7551187-Osmolar Concentration,
pubmed-meshheading:7551187-RNA, Messenger,
pubmed-meshheading:7551187-Rats,
pubmed-meshheading:7551187-Rats, Sprague-Dawley,
pubmed-meshheading:7551187-tau Proteins
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|
pubmed:year |
1995
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|
pubmed:articleTitle |
Inhibition of glutamate-induced neurotoxicity by a tau antisense oligonucleotide in primary culture of rat cerebellar granule cells.
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|
pubmed:affiliation |
Department of Biomedical Sciences and Biotechnologies, School of Medicine, University of Brescia, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
