Linked Life Data

7549783

Source:http://linkedlifedata.com/resource/pubmed/id/7549783

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1995-11-22
pubmed:abstractText
A dermatan sulfate (DS) fraction with high anticoagulant activity was isolated from the precipitate of the interaction with Concanavalin A (Con A). Under the optimum conditions found, the interaction depends absolutely on the presence of Ca++ or Mn++ ions. The pH curve of the activity showed an acute maximum at pH 4.4. The active fraction showed, in average 5.4 folds higher specific activity than the fraction that remained in the supernatant. Native DS was desulfated by acid hydrolysis: a drastic reduction in the interaction with Con A was observed during the first ten min. at 100 degrees C and a parallel decrease in the anticoagulant activity was also detected. Sulfate liberation followed the same pattern and after 30 min. at 100 degrees C no sulfate groups remain bound.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0145-5680
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
465-72
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Interaction of concanavalin A with a fraction of dermatan sulfate containing a very high anticoagulant activity.
pubmed:affiliation
Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't