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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1995-11-2
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pubmed:abstractText |
We have developed a sensitive, high-throughput bioassay to quantify angiotensin II in culture medium. Using Chinese hamster ovary cells that stably express a transfected angiotensin II receptor as target cells, we demonstrated that an agonist-stimulated myelin basic protein kinase response can be used as a basis of quantitative bioassay for angiotensin II. The assay permits detection of as little as 10 pg of angiotensin II in 1 mL of medium and offers an excellent alternative to HPLC and radioimmunoassay. This approach may also be applicable for quantification of other peptide hormones or growth factors produced by cell cultures.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin II,
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin Receptor Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Biphenyl Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/Glycogen Synthase Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Losartan,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Angiotensin,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazoles
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0736-6205
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1014-20
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:7546701-Angiotensin II,
pubmed-meshheading:7546701-Angiotensin Receptor Antagonists,
pubmed-meshheading:7546701-Animals,
pubmed-meshheading:7546701-Biological Assay,
pubmed-meshheading:7546701-Biphenyl Compounds,
pubmed-meshheading:7546701-CHO Cells,
pubmed-meshheading:7546701-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:7546701-Cricetinae,
pubmed-meshheading:7546701-Culture Media,
pubmed-meshheading:7546701-Culture Media, Serum-Free,
pubmed-meshheading:7546701-Glycogen Synthase Kinase 3,
pubmed-meshheading:7546701-Imidazoles,
pubmed-meshheading:7546701-Losartan,
pubmed-meshheading:7546701-Rats,
pubmed-meshheading:7546701-Receptors, Angiotensin,
pubmed-meshheading:7546701-Sensitivity and Specificity,
pubmed-meshheading:7546701-Tetrazoles,
pubmed-meshheading:7546701-Transfection
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pubmed:year |
1995
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pubmed:articleTitle |
Sensitive bioassay for the detection and quantification of angiotensin II in tissue culture medium.
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pubmed:affiliation |
Weis Center for Research, Danville, PA, USA.
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pubmed:publicationType |
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Technical Report
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