Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1995-10-12
pubmed:abstractText
Human immunodeficiency virus (HIV) reverse transcriptase substitutes for temperature-sensitive DNA polymerase I (Pol Its) in Escherichia coli, providing a screen for anti-HIV reverse transcriptase nucleoside analogs in bacteria. Since phosphorylation of nucleosides in E. coli is limited to thymidine and its derivatives, we coexpressed herpes simplex virus thymidine kinase, an enzyme that phosphorylates a wide variety of nucleoside analogs, together with HIV reverse transcriptase. Coexpression of herpes simplex virus thymidine kinase and HIV reverse transcriptase rendered Pol Its cells sensitive to dideoxycytidine. Studies with different nucleoside analogs indicate that this bacterial screening system is able to select and identify nucleoside analogs that specifically target HIV reverse transcriptase.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-1279198, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-1370174, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-1377646, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-14114854, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-1429652, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-1661147, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-1722328, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-188465, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-2430286, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-2460924, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-2476448, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-2479983, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-3006077, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-3327753, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-7531338, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-8218229, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-8282199, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-8387207, http://linkedlifedata.com/resource/pubmed/commentcorrection/7545249-8506308
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6563-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
A screen in Escherichia coli for nucleoside analogs that target human immunodeficiency virus (HIV) reverse transcriptase: coexpression of HIV reverse transcriptase and herpes simplex virus thymidine kinase.
pubmed:affiliation
Joseph Gottstein Memorial Cancer Research Laboratory, Department of Pathology and Biochemistry, University of Washington, Seattle 98195, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.