Linked Life Data

7543664

Source:http://linkedlifedata.com/resource/pubmed/id/7543664

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-9-13
pubmed:abstractText
Mature rat cerebellar oligodendrocytes were isolated in culture using a serum free medium and identified using anti-galactocerebroside (GalC) and OL-1 antibodies. The morphology of such oligodendrocytes changes with time in culture from a multipolar shape at about 4 days in vitro (DIV) to a monopolar shape at about 8 DIV, a transition that has been previously described only in situ. Voltage-gated ionic currents were characterized at both oligodendrocyte developmental stages using the whole cell configuration of the patch-clamp technique. No differences between these two stages were detected, both types expressed a large K+ inward rectifying current similar to that described for oligodendrocytes from other vertebrate neuronal structures. This current could play an important role in the control of oligodendrocyte resting membrane potential. Our culture system provides a valuable model, close to the situation encountered in situ, not only to study the process of oligodendrocyte maturation, but also to identify the possible interactions between oligodendrocytes and cerebellar neurons such as granular and Purkinje cells during development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0304-3940
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
190
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
191-4
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Voltage-gated ionic currents in mature oligodendrocytes isolated from rat cerebellum.
pubmed:affiliation
Laboratoire de Neurobiologie Cellulaire, UPR 9009, CNRS, Strasbourg, France.
pubmed:publicationType
Journal Article