|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0023516,
umls-concept:C0030685,
umls-concept:C0125090,
umls-concept:C0300500,
umls-concept:C0391871,
umls-concept:C0678594,
umls-concept:C0680255,
umls-concept:C0699040,
umls-concept:C0851285,
umls-concept:C1283071,
umls-concept:C1335808,
umls-concept:C1514873,
umls-concept:C1963578
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1995-9-7
|
|
pubmed:abstractText |
L-selectin mediates leukocyte rolling on vascular endothelium at sites of inflammation and lymphocyte migration to peripheral lymph nodes. L-selectin is rapidly shed from the cell surface after leukocyte activation by a proteolytic mechanism that cleaves the receptor in a membrane proximal extracellular region. This process may allow rapid leukocyte detachment from the endothelial surface before entry into tissues. In this study, the structural requirements for regulation of human L-selectin endoproteolytic release were examined through analysis of chimeric selectin molecules and mutant L-selectin receptors. The use of chimeric selectins and a cytoplasmic tail truncation mutant demonstrated that the extracellular membrane-proximal 15-amino acid region of L-selectin is required for endoproteolytic release. The introduction of alanine-scanning mutations within this membrane-proximal region did not prevent endoproteolytic release, indicating that a specific amino acid motif was not an absolute requirement for cleavage. Furthermore, alterations within the putative primary cleavage site (K283-S284) resulted in either constitutive endoproteolytic release of the receptor or inhibition of cell activation-induced shedding to variable extents. The length of the membrane-proximal region was also critical since truncations of this region completely abolished endoproteolytic release. Thus, release of L-selectin is likely to be regulated by the generation of an appropriate tertiary conformation within the membrane-proximal region of the receptor which allows recognition by a membrane-bound endoprotease with relaxed sequence specificity that cleaves the receptor at a specific distance from the plasma membrane. These observations suggest a generalized protein-processing pathway involved in the endoproteolytic release of specific transmembrane proteins which harbor widely differing primary sequences at or neighboring their cleavage sites.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1281162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1371468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1372646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1375271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1378303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1378326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1382078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1385536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1465129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1631093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1654954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1671206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1688580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1694883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1695155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1705015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1709244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1712791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1713609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1717567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1930205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1931937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1986307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-1991844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-2065357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-2165128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-2173717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-2179952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-2182714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-2466335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-2466574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-2473156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-2528680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-2551036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-2553811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-2646713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-2647302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-2829174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-3058798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-3156922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-3584977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-6277505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-6866086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-7507963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-7512970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-7518025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-7519478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-7528563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-7530761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-7534203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-7539045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-7678280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-7679710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-8021287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-8089133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-8261505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-8300647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-8407946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7543141-8424174
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0022-1007
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
182
|
|
pubmed:owner |
NLM
|
