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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
13
|
| pubmed:dateCreated |
1995-9-1
|
| pubmed:abstractText |
The Oct-2 transcription factor is expressed predominantly in B lymphocytes and has been shown previously to be important for the terminal phase of B-cell differentiation in mice. A number of genes specifically expressed in B cells contain Oct-2-binding sites in their regulatory regions. However, the analysis of expression levels of these genes in Oct-2-deficient B cells revealed that they were unaffected. Hence, there were no genes known that critically depend on Oct-2 for their expression. To understand the molecular basis for the Oct-2 effect on B-cell development, we searched for Oct-2 target genes by subtractive cDNA cloning. We show here that expression of the murine CD36 gene in B cells and macrophages requires a functional Oct-2 protein. Nuclear run-on experiments demonstrate that this gene is regulated transcriptionally by Oct-2. Moreover, CD36 levels correlated with the levels of Oct-2 expression in several mouse B-cell and macrophage cell lines. Finally, compared to wild-type and heterozygous mice, CD36 mRNA levels were markedly reduced in spleens and B-cell-enriched splenocyte fractions from oct-2-/- mice. The data identify CD36 as the first target gene critically dependent on Oct-2 for its expression. Because CD36 expression is also dependent on Oct-2 in vivo, it is a candidate gene through which Oct-2 could affect B-cell differentiation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD36,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Estradiol,
http://linkedlifedata.com/resource/pubmed/chemical/Octamer Transcription Factor-2,
http://linkedlifedata.com/resource/pubmed/chemical/Pou2f2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0890-9369
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1598-607
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7543064-Animals,
pubmed-meshheading:7543064-Antigens, CD,
pubmed-meshheading:7543064-Antigens, CD36,
pubmed-meshheading:7543064-B-Lymphocytes,
pubmed-meshheading:7543064-Base Sequence,
pubmed-meshheading:7543064-Cell Line,
pubmed-meshheading:7543064-DNA Primers,
pubmed-meshheading:7543064-DNA-Binding Proteins,
pubmed-meshheading:7543064-Estradiol,
pubmed-meshheading:7543064-Gene Expression Regulation,
pubmed-meshheading:7543064-Macrophages,
pubmed-meshheading:7543064-Mice,
pubmed-meshheading:7543064-Mice, Mutant Strains,
pubmed-meshheading:7543064-Molecular Sequence Data,
pubmed-meshheading:7543064-Octamer Transcription Factor-2,
pubmed-meshheading:7543064-RNA, Messenger,
pubmed-meshheading:7543064-Transcription, Genetic,
pubmed-meshheading:7543064-Transcription Factors
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Identification of CD36 as the first gene dependent on the B-cell differentiation factor Oct-2.
|
| pubmed:affiliation |
Zentrum für Molekulare Biologie Heidelberg, Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|