Linked Life Data

7541412

Source:http://linkedlifedata.com/resource/pubmed/id/7541412

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-8-7
pubmed:abstractText
The capacity of four subsets of CD4+ memory T cells, defined by expression of CD45RB and CD27, to provide help for B cells was examined. Larger amounts of Ig were induced by CD45RBdimCD27- cells compared with the CD45RBdimCD27+ population, whereas CD45RBbrightCD27+ or CD27- cells were poor inducers of Ig synthesis. Mitomycin C treatment, which prevents suppressive activity, markedly enhanced Ig production supported by each subset except for CD45RBbrightCD27- cells. Mitomycin C-treated CD45RBdim cells remained the most efficient inducers of Ig production, but no difference was detected between CD27+ and CD27- cells. The subsets also differed in their ability to proliferate and secrete cytokines, but these differences did not explain variations in the capacity to provide help for B cells. Both CD27- subsets exhibited decreased proliferation and uniquely secreted IL-4, with the CD45RBdimCD27- subset producing the greatest quantities of IL-4. No differences in IL-2 and IFN-gamma production were found. IL-10 secretion increased with the acquisition of the CD45RBdim phenotype and, within the CD45RBdim cells, with the loss of CD27. Staining for cytoplasmic cytokines indicated that individual populations of CD27-CD4+ helper T cells produced either IL-4 or IFN-gamma, whereas more than half of the IL-4 producers also synthesized IL-2. Finally, the different abilities of CD4+ memory T cell subsets to support B cell differentiation did not relate to variations in the expression of CD40 ligand. These results indicate that within the CD4+ memory T cell population an increase of helper activity associates with the shift from a CD45RBbright to a CD45RBdim phenotype. Within the CD45RBdim subset, the loss of CD27 is associated with a reduction of suppressive activity.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
155
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
149-62
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:7541412-Adult, pubmed-meshheading:7541412-Antigens, CD, pubmed-meshheading:7541412-Antigens, CD27, pubmed-meshheading:7541412-Antigens, CD40, pubmed-meshheading:7541412-Antigens, CD45, pubmed-meshheading:7541412-Antigens, Differentiation, B-Lymphocyte, pubmed-meshheading:7541412-B-Lymphocytes, pubmed-meshheading:7541412-CD4-Positive T-Lymphocytes, pubmed-meshheading:7541412-Cell Differentiation, pubmed-meshheading:7541412-Cells, Cultured, pubmed-meshheading:7541412-Humans, pubmed-meshheading:7541412-Immunoglobulins, pubmed-meshheading:7541412-Immunologic Memory, pubmed-meshheading:7541412-Interferon-gamma, pubmed-meshheading:7541412-Interleukin-10, pubmed-meshheading:7541412-Interleukin-2, pubmed-meshheading:7541412-Interleukin-4, pubmed-meshheading:7541412-Kinetics, pubmed-meshheading:7541412-Lymphocyte Activation, pubmed-meshheading:7541412-Lymphocyte Cooperation, pubmed-meshheading:7541412-Phenotype, pubmed-meshheading:7541412-Polymerase Chain Reaction, pubmed-meshheading:7541412-RNA, Messenger, pubmed-meshheading:7541412-T-Lymphocyte Subsets
pubmed:year
1995
pubmed:articleTitle
Expression of CD45RB and CD27 identifies subsets of CD4+ memory T cells with different capacities to induce B cell differentiation.
pubmed:affiliation
Harold C. Simmons Arthritis Research Center, University of Texas Southwestern Medical Center at Dallas 75235, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't