Linked Life Data

7541123

Source:http://linkedlifedata.com/resource/pubmed/id/7541123

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-8-2
pubmed:abstractText
Two immunoperoxidase techniques, viz. avidin-biotin complex (ABC) and peroxidase-anti-peroxidase (PAP) procedures, were applied to paraffin-wax-embedded brain-tissue sections, from brains which had been fixed in 10% formalin, to demonstrate the presence of rabies-virus antigen by light microscopy. These techniques positively identified both "viverrid" and "canid" rabies-virus antigen in tissues sections of species commonly infected with rabies virus in southern Africa, viz. the domestic dog (Canis familiaris), yellow mongoose (Cynictus penicillata), black-backed jackal (Canis mesomelas), bat-eared fox (Otocyon megalotus), cattle (Bos taurus), sheep (Ovis aries) and humans. With both of these techniques rabies-virus antigen stained as sharply demarcated, brown precipitates within the cytoplasm of neurons. The virtual absence of background staining enabled identification of fine granules of viral antigen, often referred to as "virus dust", within axons, dendrites and cytoplasm of the nerve cell body. Staining with the ABC procedure clearer, more deeply-coloured precipitates than the PAP method.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0030-2465
pubmed:author
pubmed:issnType
Print
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
183-7
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Application of immunoperoxidase techniques to formalin-fixed brain tissue for the diagnosis of rabies in southern Africa.
pubmed:affiliation
Allerton Regional Veterinary Laboratory, Pietermaritzburg, South Africa.
pubmed:publicationType
Journal Article