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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1995-7-27
|
| pubmed:abstractText |
The CD45 glycoprotein family exhibits cell-lineage-associated structural heterogeneity which is due, in part, to alternative pre-mRNA splicing. The Abelson murine leukemia (A-MuLV) preferentially transforms immature B cells that express a B-cell-specific high molecular weight CD45 isoform, called B220. However, we observed that A-MuLV-transformed cell lines are often B220- while maintaining high levels of "pan" CD45 expression. In vitro transformation of murine bone marrow revealed that the stromal microenvironment over which A-MuLV-transformed lymphoblasts are grown affected the B220 phenotype of the pre-B cells. Over a period of a few weeks, B220+ populations grown over a clonal stromal cell line gradually became B220-. However, the transition from a B220+ to B220- phenotype was dependent on the lot of fetal calf serum used. In contrast, cells grown over a heterogeneous bone marrow stroma maintained B220+ expression for long periods of time. The appearance of B220- cells in clonal B220+ populations indicated that the change in phenotype resulted in part from modulation of B220 expression. B220- B-cell lines did not express the high molecular weight CD45 RNA species indicating that the B220- phenotype was due to alternative pre-mRNA splicing. Finally, the shift from B220+ to B220- was not accompanied by changes in the stage of development of the cultures. These observations demonstrate that expression of B220 is not required for the continued proliferation of Abelson-transformed pre-B cells and is regulated by unknown environmental factors.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0882-0139
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
24
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
509-22
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7540594-Abelson murine leukemia virus,
pubmed-meshheading:7540594-Animals,
pubmed-meshheading:7540594-Antigenic Modulation,
pubmed-meshheading:7540594-Antigens, CD45,
pubmed-meshheading:7540594-Antigens, Surface,
pubmed-meshheading:7540594-B-Lymphocytes,
pubmed-meshheading:7540594-Blotting, Southern,
pubmed-meshheading:7540594-Bone Marrow Cells,
pubmed-meshheading:7540594-Cell Differentiation,
pubmed-meshheading:7540594-Cell Transformation, Viral,
pubmed-meshheading:7540594-Cells, Cultured,
pubmed-meshheading:7540594-Female,
pubmed-meshheading:7540594-Flow Cytometry,
pubmed-meshheading:7540594-Isomerism,
pubmed-meshheading:7540594-Lymphocyte Activation,
pubmed-meshheading:7540594-Mice,
pubmed-meshheading:7540594-Mice, Inbred BALB C,
pubmed-meshheading:7540594-Stromal Cells
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Bone marrow stroma-dependent modulation of CD45R isoform expression on Abelson virus transformed pre-B cells.
|
| pubmed:affiliation |
Department of Human Oncology, University of Wisconsin Medical School, Madison 53792, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|