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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1995-7-14
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pubmed:abstractText |
1. Effects of atrial natriuretic peptide (ANP) on the L-type Ca2+ channels were examined in rabbit isolated ventricular cells by use of whole-cell and cell-attached configurations of the patch clamp methods. ANP produced a concentration-dependent decrease (10-100 nM) in amplitude of a basal Ca2+ channel current. 2. The inactive ANP (methionine-oxidized ANP, 30 nM) failed to decrease the current. 3. 8-Bromo-cyclic GMP (300 microM), a potent activator of cyclic GMP-dependent protein kinase (PKG), produced the same effects on the basal Ca2+ channel current as those produced by ANP. The cyclic GMP-induced inhibition of the Ca2+ channel current was still evoked in the presence of 1-isobutyl-3-methyl-xanthine, an inhibitor of phosphodiesterase. ANP failed to produce inhibition of the Ca2+ channel current in the presence of 8-bromo-cyclic GMP. 4. In the single channel recording, ANP and 8-bromo-cyclic GMP also inhibited the activities of the L-type Ca2+ channels. Both agents decreased the open probability (NPo) without affecting the unit amplitude. 5. The present results suggest that ANP inhibits the cardiac L-type Ca2+ channel activity through the intracellular production of cyclic GMP and then activation of PKG.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-1281312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-1327579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-1373574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-1650295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-1668341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-1705030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-1891338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-1969967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2154853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2158067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2328456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2429189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2435989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2443683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2446614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2450693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2454387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2473367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2524515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2524996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2542886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2551288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2563900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2832464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2931801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2932471,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2949128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-2967190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-3017939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-3156690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-3157590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-7177773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-7678699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-8301666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7540093-8350280
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0007-1188
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
114
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1076-82
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7540093-1-Methyl-3-isobutylxanthine,
pubmed-meshheading:7540093-3-Pyridinecarboxylic acid...,
pubmed-meshheading:7540093-8-Bromo Cyclic Adenosine Monophosphate,
pubmed-meshheading:7540093-Animals,
pubmed-meshheading:7540093-Atrial Natriuretic Factor,
pubmed-meshheading:7540093-Calcium Channel Blockers,
pubmed-meshheading:7540093-Calcium Channels,
pubmed-meshheading:7540093-Cyclic GMP,
pubmed-meshheading:7540093-Heart,
pubmed-meshheading:7540093-Heart Ventricles,
pubmed-meshheading:7540093-Humans,
pubmed-meshheading:7540093-Myocardium,
pubmed-meshheading:7540093-Patch-Clamp Techniques,
pubmed-meshheading:7540093-Rabbits
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pubmed:year |
1995
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pubmed:articleTitle |
Cyclic GMP-mediated inhibition of L-type Ca2+ channel activity by human natriuretic peptide in rabbit heart cells.
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pubmed:affiliation |
Department of Pharmacology, Hokkaido University School of Medicine, Sapporo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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