Tyrosine phosphorylation of the proto-oncogene product Vav and its association with the adapter Grb2/Ash in a human leukemia cell line UT-7.

Source:http://linkedlifedata.com/resource/pubmed/id/7539782

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Authors

Sasaki K, Hirai H, Yazaki Y, Mimura T, Mitani K, Hanazono Y, Odai H

Affiliation

Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo.

Abstract

The vav proto-oncogene product (Vav) is expressed exclusively in hematopoietic cells and is reported to have guanine nucleotide exchange activity. Here we report that granulocyte-macrophage colony-stimulating factor, interleukin-3, and erythropoietin induce tyrosine phosphorylation of Vav in a human leukemia cell line UT-7. Tyrosine phosphorylation of Vav is rapid and transient; it occurs within 1 min of the stimulation and at physiological concentrations of the factors. Furthermore, we show that Vav is constitutively associated with the adapter molecule Grb2/Ash in UT-7. These data suggest that tyrosine kinases, the adapter Grb2/Ash, and the guanine nucleotide exchange factor Vav are members of a signaling pathway leading to Ras activation in hematopoietic cells.

PMID
7539782

Publication types

Research Support, Non-U.S. Gov't