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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1995-7-6
|
| pubmed:abstractText |
Chronic lymphocytic leukaemia (CLL) B cells are clones representing the mature B cell phenotype. On infection with Epstein-Barr virus (EBV) CLL cells express the EB nuclear antigen (EBNA) complex but unlike EBV-infected normal B cells they do not express LMP nor do they proliferate or immortalize. Furthermore, EBV-CLL rapidly die by apoptosis in culture. In the present study we have used the B cell growth factors interleukin 4 and antibodies to CD40 to induce activation and proliferation of EBV-infected CLL cells. Although cell numbers did not significantly increase, apoptosis was partially inhibited in CLL cells which expressed increased levels of CD23 and were activated to immunoglobulin-secreting lymphoblasts. Expression of LMP was induced by interleukin (IL)-4 and anti-CD40 in all five EBV-infected CLL samples examined. However, this did not enhance cell proliferation or induce immortalization. Further analysis showed that LMP could be detected 4-5 days after EBV infection, and that both IL-4 and anti-CD40 could independently induce LMP but that their effect was additive. These results indicate that LMP expression is dependent on B cell activation processes and that in some circumstances full latent viral gene expression is not sufficient to cause B cell immortalization.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/EBV-associated membrane antigen...,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrocortisone,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Matrix Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0887-6924
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
747-53
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7539512-Antibodies,
pubmed-meshheading:7539512-Antigens, CD,
pubmed-meshheading:7539512-Antigens, CD40,
pubmed-meshheading:7539512-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:7539512-Apoptosis,
pubmed-meshheading:7539512-B-Lymphocytes,
pubmed-meshheading:7539512-Cell Division,
pubmed-meshheading:7539512-Cell Transformation, Viral,
pubmed-meshheading:7539512-Gene Expression,
pubmed-meshheading:7539512-Herpesviridae Infections,
pubmed-meshheading:7539512-Herpesvirus 4, Human,
pubmed-meshheading:7539512-Humans,
pubmed-meshheading:7539512-Hydrocortisone,
pubmed-meshheading:7539512-Interleukin-4,
pubmed-meshheading:7539512-Leukemia, Lymphocytic, Chronic, B-Cell,
pubmed-meshheading:7539512-Lymphocyte Activation,
pubmed-meshheading:7539512-Tumor Cells, Cultured,
pubmed-meshheading:7539512-Tumor Virus Infections,
pubmed-meshheading:7539512-Viral Matrix Proteins
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Induction of latent membrane protein expression in in vitro Epstein-Barr virus-infected leukaemic B lymphocytes by interleukin 4 and antibodies to CD40.
|
| pubmed:affiliation |
Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, UK.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|