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rdf:type |
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lifeskim:mentions |
umls-concept:C0010453,
umls-concept:C0010592,
umls-concept:C0021469,
umls-concept:C0024432,
umls-concept:C0028128,
umls-concept:C0033268,
umls-concept:C0332120,
umls-concept:C0439851,
umls-concept:C0729218,
umls-concept:C1151471,
umls-concept:C1280500,
umls-concept:C1552596,
umls-concept:C1947931
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pubmed:issue |
3
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pubmed:dateCreated |
1995-6-19
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pubmed:abstractText |
Casein-elicited peritoneal macrophages from mice were cultured either alone or with interferon-gamma (IFN-gamma) and bacterial lipopolysaccharide (LPS), and the effect of cyclosporin A (CsA) and FK506 on NO2- production (due technical difficulties NO2- was taken as the index for NO) was analysed. We observed an inhibitory effect of CsA and FK506 on NO2- production. The IC50 for NO2- production by casein-elicited macrophages was 0.1 microgram/ml for CsA and 0.3 microgram/ml FK506. The effect of both drugs was dose-dependent and was more clear in non-stimulated macrophages. The presence of IFN-gamma and LPS in the culture increased NO2- production by casein-elicited macrophages and partially eliminated the inhibition exerted by CsA and FK506. Both drugs acted directly on the nitric oxide synthase (NOS), since CsA and FK506 reduced by 35% and by 17%, respectively, NOS activity in the crude cytosolic fraction. However, CsA and FK506 did not alter 14CO2 production from [1-14C]glucose, suggesting that the pentose monophosphate pathway activity was not modified. These data add new insight into the interpretation of the immunosuppressive properties of both drugs.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-1311557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-1322665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-1374612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-1380065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-1689048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-1695013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-1698860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-1711326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-1715579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-1717286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-1725930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-1847692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-1847917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-2185490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-2302181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-2445721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-2537760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-2557828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-2696179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-2735902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-2912454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-3021730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-3110273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-3242600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-3885394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-3906650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-3933532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-4400165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-6100475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-6950416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-6977503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-7181105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7538492-7504305
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
476-81
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7538492-Amino Acid Oxidoreductases,
pubmed-meshheading:7538492-Animals,
pubmed-meshheading:7538492-Cells, Cultured,
pubmed-meshheading:7538492-Cyclosporine,
pubmed-meshheading:7538492-Dose-Response Relationship, Drug,
pubmed-meshheading:7538492-Kinetics,
pubmed-meshheading:7538492-Lactates,
pubmed-meshheading:7538492-Lactic Acid,
pubmed-meshheading:7538492-Macrophages, Peritoneal,
pubmed-meshheading:7538492-Male,
pubmed-meshheading:7538492-Mice,
pubmed-meshheading:7538492-Mice, Inbred Strains,
pubmed-meshheading:7538492-Nitric Oxide,
pubmed-meshheading:7538492-Nitric Oxide Synthase,
pubmed-meshheading:7538492-Pentose Phosphate Pathway,
pubmed-meshheading:7538492-Tacrolimus
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pubmed:year |
1995
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pubmed:articleTitle |
Inhibitory effect of cyclosporin A and FK506 on nitric oxide production by cultured macrophages. Evidence of a direct effect on nitric oxide synthase activity.
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pubmed:affiliation |
Departmento de Bioquímica Médica y Biología Molecular, Facultad de Medicina, Universidad de Sevilla, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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