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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1995-6-8
|
| pubmed:abstractText |
Two distinct T-cell glycoforms of CD43 result from differential glycosylation of a single gene product in vivo. The 115 kDa glycoform carries mainly tetrasaccharides and is a pan T-cell marker, whereas the 130 kDa glycoform carries mainly hexasaccharides and is associated with T-cell activation. CD43 has been shown to play a role both in enhancing and inhibiting cell adhesion; however, the function of the individual glycoforms is unknown. We have examined the distribution and regulation of the CD43 glycoforms in a murine model of acute graft-versus-host disease (GVHD) using monoclonal antibodies (mAbs) S7 and 1B11 specific for the 115 and 130 kDa CD43 glycoforms, respectively. An increase in T-lymphocyte CD43 130 kDa expression occurred during GVHD from day 4 onwards and coincided with splenomegaly and upregulation of the beta 1-6GlcNAc transferase (C2GnT), the key enzyme responsible for the addition of complex O-glycan branching to CD43. When T-lymphocyte subsets were examined for CD43 expression, we found that in GVHD, both CD43 glycoforms were upregulated on CD4+ T cells. However, in CD8+ T cells, CD43 115 kDa was downregulated while CD43 130 kDa was dramatically upregulated, such that two distinct CD8+1B11+ T-cell subsets were observed. These data demonstrate differential expression of the CD43 glycoforms in both resting and activated CD4+ and CD8+ T cells, and suggest that glycosylation differences between the CD43 glycoforms may reflect participation in the different functions of these T-cell subsets in immune disorders in vivo.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0959-6658
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
885-93
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7537557-Acute Disease,
pubmed-meshheading:7537557-Animals,
pubmed-meshheading:7537557-Antigens, CD,
pubmed-meshheading:7537557-Antigens, CD34,
pubmed-meshheading:7537557-CD4-Positive T-Lymphocytes,
pubmed-meshheading:7537557-CD8-Positive T-Lymphocytes,
pubmed-meshheading:7537557-Carbohydrate Sequence,
pubmed-meshheading:7537557-Disease Models, Animal,
pubmed-meshheading:7537557-Down-Regulation,
pubmed-meshheading:7537557-Female,
pubmed-meshheading:7537557-Glycosylation,
pubmed-meshheading:7537557-Glycosyltransferases,
pubmed-meshheading:7537557-Graft vs Host Disease,
pubmed-meshheading:7537557-Lymphocyte Activation,
pubmed-meshheading:7537557-Mice,
pubmed-meshheading:7537557-Mice, Inbred C57BL,
pubmed-meshheading:7537557-Mice, Inbred DBA,
pubmed-meshheading:7537557-Molecular Sequence Data,
pubmed-meshheading:7537557-Molecular Weight,
pubmed-meshheading:7537557-Oligosaccharides,
pubmed-meshheading:7537557-Up-Regulation
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Differential regulation of CD43 glycoforms on CD4+ and CD8+ T lymphocytes in graft-versus-host disease.
|
| pubmed:affiliation |
Biomedical Research Centre, University of British Columbia, Vancouver, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|