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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1995-6-1
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pubmed:abstractText |
The B-cell receptor CD22 binds sialic acid linked alpha-2-6 to terminal galactose residues on N-linked oligosaccharides associated with several cell-surface glycoproteins. The first of these sialoglycoproteins to be identified was the receptor-linked phosphotyrosine phosphatase CD45, which is required for antigen/CD3-induced T-cell activation. In the present work, we examine the effect of interaction between the extracellular domain of CD45 and CD22 on T-cell activation. Using soluble CD22-immunoglobulin fusion proteins and T cells expressing wild-type and chimeric CD45 forms, we show that engagement of CD45 by soluble CD22 can modulate early T-cell signals in antigen receptor/CD3-mediated stimulation. We also show that addition of sialic acid by beta-galactoside alpha-2,6-sialyltransferase to the CD22 molecule abrogates interactions between CD22 and its ligands. Together, these observations provide direct evidence for a functional role of the interaction between the extracellular domain of CD45 and a natural ligand and suggest another regulatory mechanism for CD22-mediated ligand engagement.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-1372020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-1380033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-1438211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-1672451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-1691828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-1716973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-1717156,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-1828897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-1985119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-2061301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-2164155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-2373995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-2424993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-2523715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-2553046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-3121604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-7786324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-7901202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-8034751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-8144652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-8163475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-8191218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-8251489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-8381210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-8463234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-8463235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-8473339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-8475064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-8475387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7537381-8490965
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD22,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD45,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/CD22 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor-CD3 Complex, Antigen...,
http://linkedlifedata.com/resource/pubmed/chemical/Sialyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/beta-D-galactoside alpha...
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4026-30
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7537381-Antigens, CD,
pubmed-meshheading:7537381-Antigens, CD22,
pubmed-meshheading:7537381-Antigens, CD45,
pubmed-meshheading:7537381-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:7537381-B-Lymphocytes,
pubmed-meshheading:7537381-Calcium,
pubmed-meshheading:7537381-Cell Adhesion Molecules,
pubmed-meshheading:7537381-Cell Line,
pubmed-meshheading:7537381-Humans,
pubmed-meshheading:7537381-Inositol Phosphates,
pubmed-meshheading:7537381-Isoenzymes,
pubmed-meshheading:7537381-Kinetics,
pubmed-meshheading:7537381-Lectins,
pubmed-meshheading:7537381-Phosphorylation,
pubmed-meshheading:7537381-Phosphotyrosine,
pubmed-meshheading:7537381-Receptor-CD3 Complex, Antigen, T-Cell,
pubmed-meshheading:7537381-Sialyltransferases,
pubmed-meshheading:7537381-Type C Phospholipases,
pubmed-meshheading:7537381-Tyrosine
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pubmed:year |
1995
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pubmed:articleTitle |
Regulation of CD45 engagement by the B-cell receptor CD22.
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pubmed:affiliation |
Department of Pathology, Massachusetts General Hospital, Boston, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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