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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
1995-6-1
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pubmed:abstractText |
Replication complexes containing wild-type and RNase H-deficient p66/p51 human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) were analyzed by DNase I and S1 footprinting. While crystallography and chemical footprinting data demonstrate that 15-18 bases of primer and template occupy the DNA polymerase and RNase H active centers, enzymatic footprinting suggests that a larger portion of substrate is encompassed by the replicating enzyme. Independent of the position of DNA synthesis arrest, template nucleotides +7 to -23 and primer nucleotides -1 to -25 are nuclease resistant. On both DNA strands, position -20 remains accessible to DNase I cleavage, suggestive of an alteration in nucleic acid structure between exiting the RNase H catalytic center and leaving the C-terminal p66 domain. A model of HIV-1 RT containing an extended single-stranded template and duplex region was constructed on the basis of the structure of an RT/DNA complex. Mapping of footprint data onto this model shows consistency between biochemical and structural data, implicating a contribution from domains proximal to the catalytic centers.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-2960
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5343-56
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:7537089-Base Sequence,
pubmed-meshheading:7537089-Binding Sites,
pubmed-meshheading:7537089-DNA, Viral,
pubmed-meshheading:7537089-DNA Primers,
pubmed-meshheading:7537089-DNA Replication,
pubmed-meshheading:7537089-DNA-Directed DNA Polymerase,
pubmed-meshheading:7537089-Deoxyribonuclease I,
pubmed-meshheading:7537089-HIV Reverse Transcriptase,
pubmed-meshheading:7537089-HIV-1,
pubmed-meshheading:7537089-Models, Molecular,
pubmed-meshheading:7537089-Molecular Sequence Data,
pubmed-meshheading:7537089-Nucleic Acid Conformation,
pubmed-meshheading:7537089-Protein Conformation,
pubmed-meshheading:7537089-Protein Structure, Secondary,
pubmed-meshheading:7537089-RNA-Directed DNA Polymerase,
pubmed-meshheading:7537089-Recombinant Proteins,
pubmed-meshheading:7537089-Ribonuclease H,
pubmed-meshheading:7537089-Substrate Specificity,
pubmed-meshheading:7537089-Templates, Genetic
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pubmed:year |
1995
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pubmed:articleTitle |
An expanded model of replicating human immunodeficiency virus reverse transcriptase.
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pubmed:affiliation |
Division of Infectious Diseases, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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