Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1995-5-24
|
| pubmed:abstractText |
We prepared mAbs specific for the mouse Fas Ag (CD95) and used them to analyze the expression and apoptosis-inducing activity of the Fas Ag on murine immunocytes. Cytofluorometry of mouse bone marrow, thymus, and splenocytes using the mAbs indicated that cells of the T lineage, except for bone marrow cells, expressed Fas Ag on the surface. CD4-CD8- undifferentiated thymocytes expressed low levels of Fas Ag. Immature CD4+CD8+ thymocytes and mature CD4+CD8- and CD4-CD8+ thymocytes were highly positive for Fas Ag. CD4+CD8+ thymocytes were specifically sensitive to the apoptosis-inducing activity of anti-Fas, although CD4-CD8-, CD4+CD8-, and CD4-CD8+ thymocytes were resistant. Spleen T cells were resistant to anti-Fas, whereas they expressed Fas Ag. The superantigen, staphylococcal enterotoxin B (SEB) administered to BALB/c mice, induced clonal expansion and successive clonal deletion of spleen T cells bearing the V beta 8 TCR, which specifically reacts to SEB. Such clonal deletion of V beta 8 T cells was highly suppressed in lpr mice, which have defects in the Fas Ag gene. In SEB-administrated BALB/c mice, expression of Fas Ag was significantly enhanced on V beta 8, but not on V beta 6 T cells, which cannot react to SEB. Moreover, V beta 8 T cells in SEB-primed mice were sensitive to the cell-killing activity of anti-Fas, although V beta 6 T cells were resistant. These findings show that the expression level and apoptosis-inducing activity of Fas Ag on peripheral T cells are directly up-regulated by stimulation through the TCR in vivo.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD95,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Enterotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Superantigens,
http://linkedlifedata.com/resource/pubmed/chemical/enterotoxin B, staphylococcal
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
154
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4395-403
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7536770-Animals,
pubmed-meshheading:7536770-Antibodies, Monoclonal,
pubmed-meshheading:7536770-Antigens, CD95,
pubmed-meshheading:7536770-Antigens, Surface,
pubmed-meshheading:7536770-Bone Marrow,
pubmed-meshheading:7536770-Cell Differentiation,
pubmed-meshheading:7536770-Clonal Deletion,
pubmed-meshheading:7536770-Cricetinae,
pubmed-meshheading:7536770-Cricetulus,
pubmed-meshheading:7536770-Enterotoxins,
pubmed-meshheading:7536770-Flow Cytometry,
pubmed-meshheading:7536770-Mice,
pubmed-meshheading:7536770-Mice, Inbred BALB C,
pubmed-meshheading:7536770-Mice, Inbred C57BL,
pubmed-meshheading:7536770-Mice, Inbred DBA,
pubmed-meshheading:7536770-Mice, Mutant Strains,
pubmed-meshheading:7536770-Precipitin Tests,
pubmed-meshheading:7536770-Rats,
pubmed-meshheading:7536770-Rats, Inbred Lew,
pubmed-meshheading:7536770-Recombinant Proteins,
pubmed-meshheading:7536770-Spleen,
pubmed-meshheading:7536770-Superantigens,
pubmed-meshheading:7536770-T-Lymphocytes,
pubmed-meshheading:7536770-Thymus Gland
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Expression and function of mouse Fas antigen on immature and mature T cells.
|
| pubmed:affiliation |
Pharmaceutical Basic Research Laboratories, JT Inc., Yokohama, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|