Linked Life Data

7536665

Source:http://linkedlifedata.com/resource/pubmed/id/7536665

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-5-23
pubmed:abstractText
Detection of the neurohypophysial hormones vasopressin (AVP) and oxytocin (OT) in the testis of several species has led to the proposal that these peptides may have a physiological role in the regulation of testicular function. Therefore, we investigated whether the contractile myoid cells of rat seminiferous tubules express functional receptors for AVP or OT and, thus, constitute a target for these hormones. This study used primary cultures of purified peritubular myoid cells derived from rats both before and after puberty. By several criteria, myoid cells prepared from adult rats expressed vasopressin receptors (VPRs). We detected specific and saturable [3H]AVP binding to a single population of sites with a Kd of 7.5 nM and a binding capacity of 145 fmol/mg protein. AVP stimulated the accumulation of inositol phosphates in a dose-dependent manner with an EC50 of 1.7 nM. Cloning and sequencing of the myoid cell VPR confirmed it to be the V1a subtype of VPR. VPR expression by myoid cells is under developmental control, as the receptors are present in the adult rat, but absent before puberty. In contrast, OT receptors were not expressed at any stage of development. Peritubular myoid cells are also responsive to endothelin-1 (ET-1), which potently stimulated phosphoinositidase-C. However, unlike AVP, the ET-1 responses were observed both before and after sexual maturity, suggesting different roles for AVP and ET-1 in the control of myoid cell function. Our data establish that the myoid cells of the adult rat seminiferous tubule are a target for AVP. This indicates an additional role for AVP in the regulation of testicular function and male fertility in the adult rat.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
136
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2206-13
pubmed:dateRevised
2009-9-29
pubmed:meshHeading
pubmed-meshheading:7536665-1-Methyl-3-isobutylxanthine, pubmed-meshheading:7536665-Adenylate Cyclase, pubmed-meshheading:7536665-Aging, pubmed-meshheading:7536665-Amino Acid Sequence, pubmed-meshheading:7536665-Animals, pubmed-meshheading:7536665-Arginine Vasopressin, pubmed-meshheading:7536665-Base Sequence, pubmed-meshheading:7536665-Blotting, Northern, pubmed-meshheading:7536665-Cells, Cultured, pubmed-meshheading:7536665-Cloning, Molecular, pubmed-meshheading:7536665-Consensus Sequence, pubmed-meshheading:7536665-Cyclic AMP, pubmed-meshheading:7536665-DNA Primers, pubmed-meshheading:7536665-Forskolin, pubmed-meshheading:7536665-Gene Expression Regulation, pubmed-meshheading:7536665-Inositol Phosphates, pubmed-meshheading:7536665-Male, pubmed-meshheading:7536665-Molecular Sequence Data, pubmed-meshheading:7536665-Muscle, Smooth, pubmed-meshheading:7536665-Phosphoric Diester Hydrolases, pubmed-meshheading:7536665-Rats, pubmed-meshheading:7536665-Rats, Wistar, pubmed-meshheading:7536665-Receptors, Vasopressin, pubmed-meshheading:7536665-Seminiferous Tubules, pubmed-meshheading:7536665-Sequence Homology, Amino Acid, pubmed-meshheading:7536665-Sexual Maturation, pubmed-meshheading:7536665-Signal Transduction, pubmed-meshheading:7536665-Testis
pubmed:year
1995
pubmed:articleTitle
Rat testicular myoid cells express vasopressin receptors: receptor structure, signal transduction, and developmental regulation.
pubmed:affiliation
School of Biochemistry, University of Birmingham, United Kingdom.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't