Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-5-18
pubmed:abstractText
The cell-surface receptor for hyaluronic acid, CD44, is expressed by both normal and malignant cells. Numerous CD44 isoforms have recently been identified that are derived by alternative ribonucleic acid splicing. The expression of some CD44 isoforms has been shown to be involved in tumor progression and metastatic spread in a rat carcinoma model and in human carcinomas. In the present study, CD44 isoform expression was evaluated by reverse transcriptase-polymerase chain reaction (PCR) analysis in frozen sections derived from three samples of normal brain tissue and from 40 brain tumors, including samples of glioblastoma multiforme, anaplastic astrocytoma, low-grade astrocytoma, cerebral primitive neuroectodermal tumor, medulloblastoma, metastatic colon carcinoma, and metastatic melanoma. Normal brain tissue adjacent to the tumors was also examined in 14 of 18 glioblastomas. In all normal brain and tumor samples, with the exception of metastases from colon carcinoma, PCR analysis demonstrated one prominent product that corresponded to the CD44H hematopoietic form of CD44. Metastases from colon carcinoma demonstrated two prominent PCR amplification products corresponding to CD44H and CD44R1. These results suggest that CD44H is the predominant isoform of this protein in normal human brain tissue and in human neuroectodermal tumors of varying degrees of malignancy. The ability of CD44H to mediate tumor cell motility and invasiveness (in contrast to CD44R1) suggests that the CD44 alternative splicing pattern of neuroectoderm-derived tumors may enhance their local biological aggressiveness and intracerebral spread. The lack of expression of larger molecular weight CD44 variants by primary brain tumors may also partially explain why these tumors rarely metastasize to distant sites.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-3085
pubmed:author
pubmed:issnType
Print
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
858-63
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7536236-Alternative Splicing, pubmed-meshheading:7536236-Antigens, CD44, pubmed-meshheading:7536236-Astrocytes, pubmed-meshheading:7536236-Astrocytoma, pubmed-meshheading:7536236-Base Sequence, pubmed-meshheading:7536236-Brain, pubmed-meshheading:7536236-Brain Chemistry, pubmed-meshheading:7536236-Brain Neoplasms, pubmed-meshheading:7536236-Carcinoma, pubmed-meshheading:7536236-Carrier Proteins, pubmed-meshheading:7536236-Colonic Neoplasms, pubmed-meshheading:7536236-Glioblastoma, pubmed-meshheading:7536236-Humans, pubmed-meshheading:7536236-Melanoma, pubmed-meshheading:7536236-Molecular Sequence Data, pubmed-meshheading:7536236-Polymerase Chain Reaction, pubmed-meshheading:7536236-RNA, Messenger, pubmed-meshheading:7536236-Receptors, Cell Surface, pubmed-meshheading:7536236-Receptors, Lymphocyte Homing, pubmed-meshheading:7536236-Tumor Cells, Cultured
pubmed:year
1995
pubmed:articleTitle
Alternative RNA splicing of the hyaluronic acid receptor CD44 in the normal human brain and in brain tumors.
pubmed:affiliation
Department of Neurosurgery, University of Texas M.D. Anderson Cancer Center, Houston, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't