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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1995-5-16
|
| pubmed:databankReference | |
| pubmed:abstractText |
Expression of the human galanin gene was analysed using a 3.5-kb DNA fragment comprising the 5'-flanking sequence of the gene. This sequence contains a TATA box (ATATATA) preceded by numerous potential binding sites for transcription factors such as SP1, AP2, and NF kappa B. Three half-palindromic estrogen response elements (EREs, GGTCA) are also found at positions -1,162, -361, and -122 bp relative to the transcription start site. To localize functionally important portions of the promoter region, several shorter fragments of the galanin 5'-flanking region were placed upstream from the chloramphenicol acetyltransferase (CAT) reporter gene. In transient transfection assays, all constructs demonstrated substantial transcriptional activity in both rat glioma/mouse neuroblastoma hybrid cells (NG108-15) and Chinese hamster ovary (CHO-K1) cells. Comparison of the basal expression levels of the different constructs suggests the presence of a negative modulator between positions -1,891 and -207. When cotransfected into NG108-15 cells with the human estrogen receptor cDNA, estrogen did not induce transcription of the human galanin gene at physiological levels of estrogen receptor, although transcription was induced up to 30-fold in the presence of high levels of receptor.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Estrogens,
http://linkedlifedata.com/resource/pubmed/chemical/Gal protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Galanin,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Estrogen
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
1044-5498
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
14
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
321-9
|
| pubmed:dateRevised |
2010-9-1
|
| pubmed:meshHeading |
pubmed-meshheading:7536007-Animals,
pubmed-meshheading:7536007-Base Sequence,
pubmed-meshheading:7536007-CHO Cells,
pubmed-meshheading:7536007-Cell Line,
pubmed-meshheading:7536007-Cloning, Molecular,
pubmed-meshheading:7536007-Cricetinae,
pubmed-meshheading:7536007-DNA,
pubmed-meshheading:7536007-Estrogens,
pubmed-meshheading:7536007-Galanin,
pubmed-meshheading:7536007-Gene Expression Regulation,
pubmed-meshheading:7536007-Genes, Reporter,
pubmed-meshheading:7536007-Humans,
pubmed-meshheading:7536007-Molecular Sequence Data,
pubmed-meshheading:7536007-Peptides,
pubmed-meshheading:7536007-Promoter Regions, Genetic,
pubmed-meshheading:7536007-Protein Binding,
pubmed-meshheading:7536007-Protein Precursors,
pubmed-meshheading:7536007-Receptors, Estrogen,
pubmed-meshheading:7536007-Restriction Mapping,
pubmed-meshheading:7536007-Tumor Cells, Cultured
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Characterization of the 5'-flanking region of the human preprogalanin gene.
|
| pubmed:affiliation |
Garvan Institute of Medical Research, St. Vincent's Hospital, Darlinghurst, NSW, Australia.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|