Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
1995-5-11
|
| pubmed:abstractText |
In a previous analysis of HIV-1-specific CTLs in lymphoid organs from HIV-seropositive patients, we reported high frequencies of in vivo differentiated CTLs directed against two immunodominant regions in the central and in the C-terminal part of the HIV-1 Nef protein. The present study analyzes the epitopes recognized by CTLs in the carboxyl terminus of Nef (amino acids 182-205). In addition to several epitopes that are recognized in association with different HLA molecules (A1, A2, A25(10), B35, B52), we defined an optimal nonapeptide (190-198). This nonapeptide was recognized by CTLs down to nanomolar concentrations in the context of at least two HLA molecules, HLA-B52 and HLA-A2, including three HLA-A2 subtypes: HLA-A2.1, -A2.2, and -A2.4. We also determined the relative frequencies of effector CTLs directed against peptide 190-198 to be as high as 10(-4), as opposed to lower frequencies ranging between 5 x 10(-5) and 5 x 10(-6) observed for the other peptides recognized in the same region, thus confirming the optimal presentation of this nonapeptide in vivo. Molecular modeling of the interactions between HLA-A2.1 and Nef peptide 190-198 suggests the formation of a stable complex and allowed us to study sequence motifs that are important for the binding of the HIV-1 peptide in the pockets of the HLA-A2.1 molecule.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, nef,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A2 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-B Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-B52 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/nef Gene Products, Human...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
154
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4174-86
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7535824-Base Sequence,
pubmed-meshheading:7535824-Computer Simulation,
pubmed-meshheading:7535824-Cytotoxicity, Immunologic,
pubmed-meshheading:7535824-Epitope Mapping,
pubmed-meshheading:7535824-Epitopes,
pubmed-meshheading:7535824-Gene Products, nef,
pubmed-meshheading:7535824-HIV Antigens,
pubmed-meshheading:7535824-HIV Seropositivity,
pubmed-meshheading:7535824-HIV-1,
pubmed-meshheading:7535824-HLA-A2 Antigen,
pubmed-meshheading:7535824-HLA-B Antigens,
pubmed-meshheading:7535824-HLA-B52 Antigen,
pubmed-meshheading:7535824-Humans,
pubmed-meshheading:7535824-Immunity, Cellular,
pubmed-meshheading:7535824-Lymph Nodes,
pubmed-meshheading:7535824-Models, Molecular,
pubmed-meshheading:7535824-Molecular Sequence Data,
pubmed-meshheading:7535824-Protein Binding,
pubmed-meshheading:7535824-Spleen,
pubmed-meshheading:7535824-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:7535824-nef Gene Products, Human Immunodeficiency Virus
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
CTLs from lymphoid organs recognize an optimal HLA-A2-restricted and HLA-B52-restricted nonapeptide and several epitopes in the C-terminal region of HIV-1 Nef.
|
| pubmed:affiliation |
Laboratory for Cellular and Tissue Immunology, University Hospital Center Pitié-Salpétrière, Paris, France.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|