Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-5-2
pubmed:abstractText
Major histocompatibility complex (MHC) class I-restricted cytotoxic T lymphocytes (CTL) recognize peptide epitopes of protein antigens in a hierarchical fashion. We investigated whether proteolytic cleavage, in particular by proteasomes, is important in determining epitope hierarchy. Using highly purified 20S proteasomes, we find preferred cleavage sites directly adjacent to the N- and C-terminal ends of the immunodominant epitope of chicken ovalbumin, Ova257-264, while most of the subdominant epitope, Ova55-62, is destroyed by a major cleavage site located within this epitope. Moreover, we show that variations in amino acid sequences flanking these epitopes influence proteasomal cleavage patterns in parallel with the efficacy of their presentation. The results suggest that proteasomal cleavage within and adjacent to class I-restricted epitopes contributes to their level of presentation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1074-7613
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
289-99
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7535183-Amino Acid Sequence, pubmed-meshheading:7535183-Animals, pubmed-meshheading:7535183-Antigen Presentation, pubmed-meshheading:7535183-Cells, Cultured, pubmed-meshheading:7535183-Cysteine Endopeptidases, pubmed-meshheading:7535183-Cytotoxicity Tests, Immunologic, pubmed-meshheading:7535183-Epitopes, pubmed-meshheading:7535183-H-2 Antigens, pubmed-meshheading:7535183-Immunodominant Epitopes, pubmed-meshheading:7535183-Lymphocyte Activation, pubmed-meshheading:7535183-Mice, pubmed-meshheading:7535183-Mice, Inbred C57BL, pubmed-meshheading:7535183-Microscopy, Confocal, pubmed-meshheading:7535183-Molecular Sequence Data, pubmed-meshheading:7535183-Multienzyme Complexes, pubmed-meshheading:7535183-Ovalbumin, pubmed-meshheading:7535183-Peptide Fragments, pubmed-meshheading:7535183-Proteasome Endopeptidase Complex
pubmed:year
1995
pubmed:articleTitle
Contribution of proteasome-mediated proteolysis to the hierarchy of epitopes presented by major histocompatibility complex class I molecules.
pubmed:affiliation
Max Planck Institut für Immunbiologie, Freiburg, Federal Republic of Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't