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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1995-4-21
|
| pubmed:abstractText |
This study demonstrates post-mortem autolytic alterations in the skin at cellular and subcellular levels and identifies parameters which may assist in determining the time of death in the first few hours post-mortem. Serial skin samples from the ventral surface of the arm were taken at intervals of 3, 6, 9 and 12 h after death in 29 subjects of various ages, with no signs of skin disease; causes of death were various. Three types of tests were performed: cytochemical (hematoxylin-eosin and alcian-PAS), immunohistochemical (S-100, CEA, Cytokeratin, ASM) and ultrastructural (electron microscopy). Electron microscopy proved useful for identifying transformations which were found to be specific for each chronological step considered: reduction of intracellular glycogen in clear cells and reduction of secretory granules in dark cells are typical signs of the first stage (3 h) after death; mitochondrial dilatation and rarefaction of cristae in clear and dark cells are typical of the second stage (6 h); rarefaction of microvilli in dark and clear cells is a sign of the last stage (12 h). Cytochemistry and immunohistochemistry supply useful information--not for all the chronological stage considered here, but for individual phases (3 h for hematoxylin-eosin and 6 h for alcian-PAS). However, it is particularly important to use the results from all such techniques simultaneously, so that the question of the exact time of death within the first 12 h post-mortem may be more accurately answered.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0937-9827
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
107
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
132-40
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7534470-Actins,
pubmed-meshheading:7534470-Aged,
pubmed-meshheading:7534470-Autolysis,
pubmed-meshheading:7534470-Carcinoembryonic Antigen,
pubmed-meshheading:7534470-Cell Membrane,
pubmed-meshheading:7534470-Cytoplasm,
pubmed-meshheading:7534470-Female,
pubmed-meshheading:7534470-Histocytochemistry,
pubmed-meshheading:7534470-Humans,
pubmed-meshheading:7534470-Immunohistochemistry,
pubmed-meshheading:7534470-Keratins,
pubmed-meshheading:7534470-Male,
pubmed-meshheading:7534470-Microscopy, Electron,
pubmed-meshheading:7534470-Middle Aged,
pubmed-meshheading:7534470-Mitochondria,
pubmed-meshheading:7534470-S100 Proteins,
pubmed-meshheading:7534470-Sweat Glands,
pubmed-meshheading:7534470-Time Factors
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Morphology of sweat glands in determining time of death.
|
| pubmed:affiliation |
Istituto di Medicina Legale, Università di Ancona, Italy.
|
| pubmed:publicationType |
Journal Article
|