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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1995-4-17
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pubmed:abstractText |
The tylosin producer Streptomyces fradiae contains four known resistance genes, two of which (tlrA and tlrD) encode methyltransferases that act on ribosomal RNA at a common site. Expression of tlrA is regulated via transcriptional attenuation. A short transcript, only 411 nucleotides long, terminates 27 nucleotides into the methylase-coding sequence in the uninduced state. Induction of tlrA is proposed to involve a ribosome-mediated conformational change within the mRNA leader that allows transcription to continue beyond the attenuation site, resulting in a transcript about 1450 nucleotides long. Transplantation of tlrD and/or tlrA into Streptomyces albus revealed that the induction specificity of tlrA depends upon the state of the ribosomes and is significantly altered in strains also expressing tlrD.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0950-382X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:geneSymbol |
tlrA,
tlrD
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
833-42
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7534372-Base Sequence,
pubmed-meshheading:7534372-Binding Sites,
pubmed-meshheading:7534372-Carbohydrate Sequence,
pubmed-meshheading:7534372-Chromosome Mapping,
pubmed-meshheading:7534372-DNA, Bacterial,
pubmed-meshheading:7534372-DNA Primers,
pubmed-meshheading:7534372-Drug Resistance, Microbial,
pubmed-meshheading:7534372-Genes, Bacterial,
pubmed-meshheading:7534372-Methyltransferases,
pubmed-meshheading:7534372-Molecular Sequence Data,
pubmed-meshheading:7534372-RNA, Bacterial,
pubmed-meshheading:7534372-RNA, Messenger,
pubmed-meshheading:7534372-Ribosomes,
pubmed-meshheading:7534372-Streptomyces,
pubmed-meshheading:7534372-Transcription, Genetic,
pubmed-meshheading:7534372-Tylosin
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pubmed:year |
1994
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pubmed:articleTitle |
Transcriptional attenuation control of the tylosin-resistance gene tlrA in Streptomyces fradiae.
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pubmed:affiliation |
Department of Biochemistry, University of Leicester, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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