|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
1995-4-19
|
|
pubmed:abstractText |
1. These studies examine the effect of retinoids on interleukin 1 beta (IL-1 beta)-induced nitric oxide synthase (NOS) activity in cultured rat aortic vascular smooth muscle (VSM) cells and isolated rat aortic rings. 2. All-trans-retinoic acid (all-trans-RA, 0.1-10 microM) and its active analogues produced concentration-dependent inhibition of IL-1 beta (0.1-10 ng ml-1)-induced nitrite production in cultured VSM cells. In contrast, the inactive retinoid, Ro 14-6113 (0.1-10 microM), had no effect on IL-1 beta-induced nitrite production. 3. Since some of the actions of retinoids are mediated by induction of transforming growth factor beta (TGF-beta), its effect on inducible NOS activity in VSM cells was examined. TGF-beta produced concentration-dependent (0.1-10 ng ml-1) inhibition of IL-1 beta-induced nitrite production and the maximum effect (approximately 90% inhibition) was significantly greater than that seen with all-trans-RA (approximately 70% with 10 microM). However, an anti-TGF-beta antibody (50 micrograms ml-1) which blocked the effect of exogenous TGF-beta (5 ng ml-1) did not significantly reverse the inhibitory action of all-trans-RA (10 microM). 4. In addition to inhibiting IL-1 beta-induced nitrite production, all-trans-RA (10 microM) reduced substantially inducible NOS mRNA and protein levels in IL-1 beta-induced VSM cells (P < 0.01). 5. Incubation of isolated rat aortic rings with IL-1 beta (10 ng ml-1) caused a progressive resistance of the rings to the vasoconstrictor action of phenylephrine (10 nM to 10 microM). This effect was abolished by the addition of the nitric oxide synthase inhibitor L-NG-monomethyl-L-arginine (L-NMMA, 1 mM). All trans-RA (10 micro M) also markedly and significantly reversed this IL-1p-induced vascular hyporeactivity(P<0.01).6. These data show that all-trans-RA and other active retinoids are able to block cytokine-stimulated expression of inducible NOS in cultured VSM cells and isolated aortic rings.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1282320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1309942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1310260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1327576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1330076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1330304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1338435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1361398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1371674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1372433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1375656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1385162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1460122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1648728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1671393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1702214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1852778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-1924551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-2134349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-2174116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-2176152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-2497467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-3024708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-6222758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-6930676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-7506668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-7680540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-7680561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-7684434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-7689053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-7690141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-7902846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-8120450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-8368284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-8428468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7534188-8515790
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0007-1188
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
113
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1448-54
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:7534188-Amino Acid Oxidoreductases,
pubmed-meshheading:7534188-Animals,
pubmed-meshheading:7534188-Aorta, Thoracic,
pubmed-meshheading:7534188-Base Sequence,
pubmed-meshheading:7534188-Blotting, Western,
pubmed-meshheading:7534188-Interleukin-1,
pubmed-meshheading:7534188-Male,
pubmed-meshheading:7534188-Molecular Sequence Data,
pubmed-meshheading:7534188-Muscle, Smooth, Vascular,
pubmed-meshheading:7534188-Nitric Oxide,
pubmed-meshheading:7534188-Nitric Oxide Synthase,
pubmed-meshheading:7534188-Polymerase Chain Reaction,
pubmed-meshheading:7534188-RNA-Directed DNA Polymerase,
pubmed-meshheading:7534188-Rats,
pubmed-meshheading:7534188-Rats, Wistar,
pubmed-meshheading:7534188-Retinoids,
pubmed-meshheading:7534188-Transforming Growth Factor beta,
pubmed-meshheading:7534188-Tretinoin
|
|
pubmed:year |
1994
|
|
pubmed:articleTitle |
Inhibition of nitric oxide synthesis in vascular smooth muscle by retinoids.
|
|
pubmed:affiliation |
Department of Clinical Pharmacology, Royal Postgraduate Medical School, London.
|
|
pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|
