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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1995-4-7
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pubmed:databankReference |
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pubmed:abstractText |
We have isolated a cDNA (SKv1.1) encoding a Shaker-related K+ channel from an adult cDNA library of the human parasitic trematode Schistosoma mansoni. The deduced amino acid sequence (512 aa, 56.5 kDa) contains 6 putative membrane-spanning domains (S1-S6) and a pore-forming domain (H5). SKv1.1 is grouped in the Shaker family, but forms a unique branch within this family, on the basis of dendrogram analysis. SKv1.1 shows significant sequence identity with most other Shaker channels, with 64-74% identity in the core region (S1-S6). It has the highest sequence identity with the K+ channel (Ak01a) from Aplysia. Northern blot analysis detected a single primary transcript of 2.8 kb. Southern blot analysis indicated that SKv1.1 is present as a single copy in the genomic DNA of S. mansoni. Expression of SKv1.1 in Xenopus oocytes produced a rapidly activating and inactivating outward K+ current which is highly sensitive to 4-aminopyridine, but is insensitive to tetraethylammonium, mast cell degranulating peptide, dendrotoxin and charybdotoxin. The presence of a Shaker homologue in Schistosoma suggests that Sh subfamilies may exist in other lower invertebrates as well as platyhelminths.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0031-1820
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
110 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
171-80
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7533899-Amino Acid Sequence,
pubmed-meshheading:7533899-Animals,
pubmed-meshheading:7533899-Base Sequence,
pubmed-meshheading:7533899-Cloning, Molecular,
pubmed-meshheading:7533899-DNA, Complementary,
pubmed-meshheading:7533899-Female,
pubmed-meshheading:7533899-Gene Expression,
pubmed-meshheading:7533899-Gene Library,
pubmed-meshheading:7533899-Genes, Helminth,
pubmed-meshheading:7533899-Ion Channel Gating,
pubmed-meshheading:7533899-Molecular Sequence Data,
pubmed-meshheading:7533899-Nucleic Acid Hybridization,
pubmed-meshheading:7533899-Oocytes,
pubmed-meshheading:7533899-Potassium,
pubmed-meshheading:7533899-Potassium Channels,
pubmed-meshheading:7533899-RNA,
pubmed-meshheading:7533899-Schistosoma mansoni,
pubmed-meshheading:7533899-Selection, Genetic,
pubmed-meshheading:7533899-Sequence Analysis, DNA,
pubmed-meshheading:7533899-Sequence Homology, Amino Acid,
pubmed-meshheading:7533899-Shaker Superfamily of Potassium Channels,
pubmed-meshheading:7533899-Xenopus
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pubmed:year |
1995
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pubmed:articleTitle |
Cloning and functional expression of a Shaker-related voltage-gated potassium channel gene from Schistosoma mansoni (Trematoda: Digenea).
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pubmed:affiliation |
Department of Pharmacology and Toxicology, Michigan State University, East Lansing 48824.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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