Linked Life Data

7533529

Source:http://linkedlifedata.com/resource/pubmed/id/7533529

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1995-4-13
pubmed:abstractText
It has been shown that the gene ERG in 21q22 is rearranged in the t(16;21)(p11;q22) associated with acute myeloid leukemia (AML). ERG is a member of the ETS gene family and is fused with EWS in a subset of Ewing's sarcomas. EWS in 22q12 has a very high homology with FUS (also called TLS) in 16p11; the latter gene is rearranged in the t(12;16)(q13;p11) that characterizes myxoid liposarcoma. To investigate whether FUS is involved in the t(16;21) of AML, we used the Southern blot technique and polymerase chain reaction (PCR) to examine the bone marrow of a 3-year-old boy with a t(16;21)(p11;q22)-positive AML. Hybridization of Southern blot filters containing digested DNA with probes for FUS and ERG showed both germline and aberrant fragments. Using specific primers for the 5' part of FUS and the 3' part of ERG, we amplified a 4.4 kb genomic FUS/ERG DNA fragment from the leukemic sample. In a second PCR experiment, in which we used primers upstream of the 5' part of ERG and downstream of the 3' part of FUS, a 5.6 kb fragment was amplified. Blotting and hybridization with specific probes for FUS and ERG revealed that the amplified fragments consisted of FUS/ERG and ERG/FUS hybrid DNA. Both PCR fragments, when used as probes, detected germline ERG and FUS as well as aberrant fragments on Southern blot filters. The results suggest that the t(16;21) in AML leads to rearrangement and fusion of the FUS and ERG genes.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1045-2257
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:geneSymbol
ERG
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
256-62
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:7533529-Base Sequence, pubmed-meshheading:7533529-Blotting, Southern, pubmed-meshheading:7533529-Child, Preschool, pubmed-meshheading:7533529-Chromosomes, Human, Pair 16, pubmed-meshheading:7533529-Chromosomes, Human, Pair 22, pubmed-meshheading:7533529-DNA Probes, pubmed-meshheading:7533529-Gene Rearrangement, pubmed-meshheading:7533529-Heterogeneous-Nuclear Ribonucleoproteins, pubmed-meshheading:7533529-Humans, pubmed-meshheading:7533529-Leukemia, Myeloid, Acute, pubmed-meshheading:7533529-Male, pubmed-meshheading:7533529-Molecular Sequence Data, pubmed-meshheading:7533529-Neoplasm Proteins, pubmed-meshheading:7533529-RNA-Binding Protein EWS, pubmed-meshheading:7533529-RNA-Binding Protein FUS, pubmed-meshheading:7533529-Ribonucleoproteins, pubmed-meshheading:7533529-Translocation, Genetic
pubmed:year
1994
pubmed:articleTitle
Fusion of the FUS gene with ERG in acute myeloid leukemia with t(16;21)(p11;q22).
pubmed:affiliation
Department of Clinical Genetics, University Hospital, Lund, Sweden.
pubmed:publicationType
Journal Article, Comparative Study, Case Reports, Research Support, Non-U.S. Gov't