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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1995-3-28
|
| pubmed:abstractText |
The authors describe the development and the application of polymerase chain reaction (PCR) in the detection of hepatitis C virus. Based on the sequence data available in the literature, a conservative motif at the 5' end of the virus genome has been selected for amplification. Using the flanking synthetic oligonucleotides the specificity of the highly sensitive RNA-PCR assay was proved by serologically positive and negative serum samples. HCV-RNA-PCR studies were applied to start interferon treatment in patients from National Institute of Rheumatology and Physiotherapy (Budapest) with anti-HCV positive serum samples and this method was used to evaluate the efficacy of interferon therapy, as well.
|
| pubmed:language |
hun
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0030-6002
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
12
|
| pubmed:volume |
136
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
339-41
|
| pubmed:dateRevised |
2009-10-21
|
| pubmed:meshHeading | |
| pubmed:year |
1995
|
| pubmed:articleTitle |
[Detection of hepatitis C viral RNA by polymerase chain reaction].
|
| pubmed:affiliation |
Semmelweis Orvostudományi Egyetem, Biológiai Intézet, Budapest.
|
| pubmed:publicationType |
Journal Article,
English Abstract
|