Linked Life Data

7532466

Source:http://linkedlifedata.com/resource/pubmed/id/7532466

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-3-30
pubmed:abstractText
GB24 is a mouse monoclonal antibody raised against a common trophoblast-lymphocyte cross-reactive antigen. GB24 detects the membrane cofactor protein (MCP, CD46), a member of the complement regulatory protein family, which serves as a cofactor for factor 1 mediated cleavage of C3b. This study investigated the reactivity of GB24 on 38 breast carcinomas and 34 normal/benign breast tissues by immunochemistry as well as the reactivity of F2B7-2, an antibody specific to the decay accelerating factor (DAF, CD55) of the complement. GB24 staining was present on both normal tissue and benign lesions, but very strong diffuse reactivity was observed on carcinomas. This reactivity increased with the tumor grade. By contrast, malignant tumor cells lacked DAF expression. F2B7-2 antibody reacted weakly with benign epithelial cells. Results were studied by computer assisted image analysis to accurately define the mean optical densities. The densitometric analysis of MCP positive carcinomas showed a high intensity of the staining. Expression of MCP and DAF on MCF-7 cell lines was analyzed by flow cytometry. MCF-7 cell lines were strongly stained by mAb GB24 only. These data suggest that selectively enhanced expression of the antigen recognized by GB24 is associated with malignant breast disorders. This high expression, which may reflect a protective mechanism by which tumor cells survive complement activation, may prove useful as a marker of malignant transformation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0167-6806
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
213-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7532466-Adenocarcinoma, pubmed-meshheading:7532466-Antibodies, Monoclonal, pubmed-meshheading:7532466-Antibody Specificity, pubmed-meshheading:7532466-Antigens, CD, pubmed-meshheading:7532466-Antigens, CD46, pubmed-meshheading:7532466-Antigens, CD55, pubmed-meshheading:7532466-Antigens, Neoplasm, pubmed-meshheading:7532466-Breast, pubmed-meshheading:7532466-Breast Neoplasms, pubmed-meshheading:7532466-Carcinoma, Ductal, Breast, pubmed-meshheading:7532466-Carcinoma, Intraductal, Noninfiltrating, pubmed-meshheading:7532466-Carcinoma, Lobular, pubmed-meshheading:7532466-Cell Differentiation, pubmed-meshheading:7532466-Complement System Proteins, pubmed-meshheading:7532466-Cross Reactions, pubmed-meshheading:7532466-Epithelium, pubmed-meshheading:7532466-Female, pubmed-meshheading:7532466-Fibroadenoma, pubmed-meshheading:7532466-Fibrocystic Breast Disease, pubmed-meshheading:7532466-Gene Expression, pubmed-meshheading:7532466-Humans, pubmed-meshheading:7532466-Membrane Glycoproteins, pubmed-meshheading:7532466-Neoplasm Proteins, pubmed-meshheading:7532466-Trophoblasts, pubmed-meshheading:7532466-Tumor Cells, Cultured, pubmed-meshheading:7532466-Tumor Markers, Biological
pubmed:year
1994
pubmed:articleTitle
High expression of the antigen recognized by the monoclonal antibody GB24 on human breast carcinomas: a preventive mechanism of malignant tumor cells against complement attack?
pubmed:affiliation
INSERM U364, University of Nice, France.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't