Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1995-3-8
pubmed:abstractText
Effects of Pb and several other metal ions on various distinct types of voltage-, receptor- and Ca-activated ion channels have been investigated in cultured N1E-115 mouse neuroblastoma cells. Experiments were performed using the whole-cell voltage clamp and single-channel patch clamp techniques. External superfusion of nanomolar to submillimolar concentrations of Pb causes multiple effects on ion channels. Barium current through voltage-activated Ca channels is blocked by micromolar concentrations of Pb, whereas voltage-activated Na current appears insensitive. Neuronal type nicotinic acetylcholine receptor-activated ion current is blocked by nanomolar concentrations of Pb and this block is reversed at micromolar concentrations. Serotonin 5-HT3 receptor-activated ion current is much less sensitive to Pb. In addition, external superfusion with micromolar concentrations of Pb as well as of Cd and aluminum induces inward current, associated with the direct activation of nonselective cation channels by these metal ions. In excised inside-out membrane patches of neuroblastoma cells, micromolar concentrations of Ca activate small (SK) and big (BK) Ca-activated K channels. Internally applied Pb activates SK and BK channels more potently than Ca, whereas Cd is approximately equipotent to Pb with respect to SK channel activation, but fails to activate BK channels. The results show that metal ions cause distinct, selective effects on the various types of ion channels and that metal ion interaction sites of ion channels may be highly selective for particular metal ions.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-1488279, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-1488280, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-1650279, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-1656334, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-1660583, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-1671748, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-1689669, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-1690461, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-1692342, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-1846400, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-2443646, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-2482354, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-2483110, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-2605523, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-2771056, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-2771873, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-2895506, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-3715885, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-3787636, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-386513, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-4400294, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-6122211, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-6270629, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-6320979, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-6525509, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-6703041, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-671297, http://linkedlifedata.com/resource/pubmed/commentcorrection/7531139-6737462
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0091-6765
pubmed:author
pubmed:issnType
Print
pubmed:volume
102 Suppl 3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
153-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Metal interactions with voltage- and receptor-activated ion channels.
pubmed:affiliation
Research Institute of Toxicology, Utrecht University, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't