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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1 Pt 1
|
| pubmed:dateCreated |
1995-2-28
|
| pubmed:abstractText |
Secretogranin II (SG-II) is an acidic 86-kDa protein found in high abundance in the anterior pituitary gland. In the present studies, we investigated the secretion and the localization of SG-II using pituitary cells from female rats at all stages of the estrous cycle. Double immunofluorescence staining revealed that SG-II immunoreactivity was localized in low abundance in about half of all pituitary cells and in high abundance in all of the luteinizing hormone (LH)-immunoreactive cells (which represent approximately 5% of all pituitary cells). Using a reverse hemolytic plaque assay for measurement of SG-II release from individual pituitary cells in culture, we found that SG-II secretion was strongly stimulated by gonadotropin-releasing hormone in a dose-related fashion, and the amount of SG-II secretion was also related to the stage of the estrous cycle: it was highest at proestrus and lowest at estrus. SG-II plaque assay followed by LH immunofluorescence staining further revealed that all the SG-II-secreting cells contained LH immunoreactivity. At proestrus all the LH-immunoreactive cells secreted SG-II, whereas another days of the estrous cycle only a fraction of them did so. Thus our findings demonstrate a striking resemblance between SG-II and LH with regard to cell localization and secretory regulation.
|
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0002-9513
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
268
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
E145-52
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| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7530910-Animals,
pubmed-meshheading:7530910-Chromogranins,
pubmed-meshheading:7530910-Estrus,
pubmed-meshheading:7530910-Female,
pubmed-meshheading:7530910-Fluorescent Antibody Technique,
pubmed-meshheading:7530910-Hemolytic Plaque Technique,
pubmed-meshheading:7530910-Immune Sera,
pubmed-meshheading:7530910-Luteinizing Hormone,
pubmed-meshheading:7530910-Pituitary Gland, Anterior,
pubmed-meshheading:7530910-Proteins,
pubmed-meshheading:7530910-Rats,
pubmed-meshheading:7530910-Rats, Sprague-Dawley,
pubmed-meshheading:7530910-Staining and Labeling,
pubmed-meshheading:7530910-Tissue Distribution
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Measurement of secretogranin II release from individual adenohypophysial gonadotropes.
|
| pubmed:affiliation |
Department of Physiology and Biophysics, University of Alabama at Birmingham 35294.
|
| pubmed:publicationType |
Journal Article
|