Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1995-2-17
|
pubmed:abstractText |
Allogeneic transplantation of granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood progenitor cells (PBPC) appears to be an attractive alternative to allogeneic bone marrow transplantation (BMT). However, because vast amounts of potentially graft-vs.-host-reactive T cells are transfused with PBPC grafts, the use of PBPC in the allogeneic setting may be associated with an increased incidence or severity of graft-vs.-host disease (GVHD). To evaluate strategies for prevention of GVHD after PBPC allografting, we have studied T cell depletion (TCD) of G-CSF-mobilized PBPC samples harvested from six healthy donors and from five patients scheduled for autologous PBPC transplantation. Three approaches (CAMPATH-1 plus autologous complement [C], immunomagnetic CD34+ cell selection, and biotin-avidin-mediated CD34+ cell selection) were compared. TCD of PBPC samples with the monoclonal antibody (MAb) CAMPATH-1 plus autologous C resulted in a median elimination of 2.16 log CD3+ T cells, whereas 39% of CD56+ natural killer (NK) cells and 56% of CD34+ progenitor cells were recovered. TCD by CD34+ cell selection with the Isolex (Baxter, Munich, Germany) or Ceprate (CellPro, Bothell, WA) devices achieved median depletions (Isolex vs. Ceprate) of 4.04 vs. 3.12 log T cells and > 5 vs. 3.27 log NK cells while allowing the recovery of 36 vs. 27% CD34+ cells. The median purity of CD34+ cells in the final product was 1.7 (CAMPATH-1), 94 (Isolex), and 65% (Ceprate). We conclude that all methods tested effectively deplete T cells from PBPC preparations harvested from healthy donors. Whereas immunomagnetic CD34+ selection is most effective in terms of elimination of T cells, the less intensive T and NK cell depletions achieved with CAMPATH-1 might be advantageous with regard to retaining engraftment potential and graft-vs.-leukemia (GVL) activity of PBPC allografts.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal, Humanized,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Avidin,
http://linkedlifedata.com/resource/pubmed/chemical/Biotin,
http://linkedlifedata.com/resource/pubmed/chemical/CD52 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Complement System Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte Colony-Stimulating...,
http://linkedlifedata.com/resource/pubmed/chemical/alemtuzumab
|
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
0301-472X
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
23
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
147-54
|
pubmed:dateRevised |
2011-11-17
|
pubmed:meshHeading |
pubmed-meshheading:7530212-Adolescent,
pubmed-meshheading:7530212-Adult,
pubmed-meshheading:7530212-Aged,
pubmed-meshheading:7530212-Antibodies, Monoclonal,
pubmed-meshheading:7530212-Antibodies, Monoclonal, Humanized,
pubmed-meshheading:7530212-Antibodies, Neoplasm,
pubmed-meshheading:7530212-Antigens, CD,
pubmed-meshheading:7530212-Antigens, CD34,
pubmed-meshheading:7530212-Antigens, Neoplasm,
pubmed-meshheading:7530212-Avidin,
pubmed-meshheading:7530212-Biotin,
pubmed-meshheading:7530212-Blood Component Removal,
pubmed-meshheading:7530212-Complement System Proteins,
pubmed-meshheading:7530212-Glycoproteins,
pubmed-meshheading:7530212-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:7530212-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:7530212-Humans,
pubmed-meshheading:7530212-Immunomagnetic Separation,
pubmed-meshheading:7530212-Immunophenotyping,
pubmed-meshheading:7530212-Middle Aged,
pubmed-meshheading:7530212-T-Lymphocytes
|
pubmed:year |
1995
|
pubmed:articleTitle |
G-CSF-mobilized peripheral blood progenitor cells for allogeneic transplantation: comparison of T cell depletion strategies using different CD34+ selection systems or CAMPATH-1.
|
pubmed:affiliation |
Second Department of Medicine, University of Kiel, Germany.
|
pubmed:publicationType |
Journal Article
|