Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-2-17
pubmed:abstractText
Allogeneic transplantation of granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood progenitor cells (PBPC) appears to be an attractive alternative to allogeneic bone marrow transplantation (BMT). However, because vast amounts of potentially graft-vs.-host-reactive T cells are transfused with PBPC grafts, the use of PBPC in the allogeneic setting may be associated with an increased incidence or severity of graft-vs.-host disease (GVHD). To evaluate strategies for prevention of GVHD after PBPC allografting, we have studied T cell depletion (TCD) of G-CSF-mobilized PBPC samples harvested from six healthy donors and from five patients scheduled for autologous PBPC transplantation. Three approaches (CAMPATH-1 plus autologous complement [C], immunomagnetic CD34+ cell selection, and biotin-avidin-mediated CD34+ cell selection) were compared. TCD of PBPC samples with the monoclonal antibody (MAb) CAMPATH-1 plus autologous C resulted in a median elimination of 2.16 log CD3+ T cells, whereas 39% of CD56+ natural killer (NK) cells and 56% of CD34+ progenitor cells were recovered. TCD by CD34+ cell selection with the Isolex (Baxter, Munich, Germany) or Ceprate (CellPro, Bothell, WA) devices achieved median depletions (Isolex vs. Ceprate) of 4.04 vs. 3.12 log T cells and > 5 vs. 3.27 log NK cells while allowing the recovery of 36 vs. 27% CD34+ cells. The median purity of CD34+ cells in the final product was 1.7 (CAMPATH-1), 94 (Isolex), and 65% (Ceprate). We conclude that all methods tested effectively deplete T cells from PBPC preparations harvested from healthy donors. Whereas immunomagnetic CD34+ selection is most effective in terms of elimination of T cells, the less intensive T and NK cell depletions achieved with CAMPATH-1 might be advantageous with regard to retaining engraftment potential and graft-vs.-leukemia (GVL) activity of PBPC allografts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal, http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal, Humanized, http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Neoplasm, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm, http://linkedlifedata.com/resource/pubmed/chemical/Avidin, http://linkedlifedata.com/resource/pubmed/chemical/Biotin, http://linkedlifedata.com/resource/pubmed/chemical/CD52 antigen, http://linkedlifedata.com/resource/pubmed/chemical/Complement System Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte Colony-Stimulating..., http://linkedlifedata.com/resource/pubmed/chemical/alemtuzumab
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0301-472X
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
147-54
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:7530212-Adolescent, pubmed-meshheading:7530212-Adult, pubmed-meshheading:7530212-Aged, pubmed-meshheading:7530212-Antibodies, Monoclonal, pubmed-meshheading:7530212-Antibodies, Monoclonal, Humanized, pubmed-meshheading:7530212-Antibodies, Neoplasm, pubmed-meshheading:7530212-Antigens, CD, pubmed-meshheading:7530212-Antigens, CD34, pubmed-meshheading:7530212-Antigens, Neoplasm, pubmed-meshheading:7530212-Avidin, pubmed-meshheading:7530212-Biotin, pubmed-meshheading:7530212-Blood Component Removal, pubmed-meshheading:7530212-Complement System Proteins, pubmed-meshheading:7530212-Glycoproteins, pubmed-meshheading:7530212-Granulocyte Colony-Stimulating Factor, pubmed-meshheading:7530212-Hematopoietic Stem Cell Transplantation, pubmed-meshheading:7530212-Humans, pubmed-meshheading:7530212-Immunomagnetic Separation, pubmed-meshheading:7530212-Immunophenotyping, pubmed-meshheading:7530212-Middle Aged, pubmed-meshheading:7530212-T-Lymphocytes
pubmed:year
1995
pubmed:articleTitle
G-CSF-mobilized peripheral blood progenitor cells for allogeneic transplantation: comparison of T cell depletion strategies using different CD34+ selection systems or CAMPATH-1.
pubmed:affiliation
Second Department of Medicine, University of Kiel, Germany.
pubmed:publicationType
Journal Article