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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-2-21
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pubmed:abstractText |
The modulation of cyclic AMP dependent neurosteroidogenesis was studied in minces prepared from the cerebral cortex of adult rat. Forskolin or dibutyryl-cyclic AMP enhanced pregnenolone and progesterone production in a time and dose-dependent manner. The forskolin effect was mimicked by the cyclic AMP phosphodiesterase inhibitor isobutyl-methyl-xanthine, but not by the adenylate cyclase inactive forskolin analogue 1,9,dideoxy-forskolin. 4'-Chloro-diazepam, a high affinity ligand for the mitochondrial diazepam binding inhibitor (DBI) receptor, also elicited a time dependent increase in steroidogenesis. The forskolin and the 4'-chloro-diazepam stimulated pregnenolone increase was prevented by preexposing the rat brain cortical minces to 1-(2-chlorophenyl)-N-methyl-N-(1-methyl-propyl)-3-isoquinoline carboxamide (PK 11195), a high affinity ligand for the mitochondrial DBI receptor endowed with antagonistic properties. The protein synthesis inhibitor cycloheximide prevented the forskolin and 4'-chloro-diazepam stimulation of pregnenolone formation. In brain cortical minces of adrenalectomised/orchiectomised rats dibutyryl-cyclic AMP increased both pregnenolone and progesterone formation, while forskolin only increased progesterone. These data show that cyclic AMP enhances brain steroidogenesis by acting on a labile protein substrate which interacts with the mitochondrial DBI receptor.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,9-dideoxyforskolin,
http://linkedlifedata.com/resource/pubmed/chemical/1-Methyl-3-isobutylxanthine,
http://linkedlifedata.com/resource/pubmed/chemical/4'-chlorodiazepam,
http://linkedlifedata.com/resource/pubmed/chemical/Benzodiazepinones,
http://linkedlifedata.com/resource/pubmed/chemical/Bucladesine,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/Isoquinolines,
http://linkedlifedata.com/resource/pubmed/chemical/PK 11195,
http://linkedlifedata.com/resource/pubmed/chemical/Pregnenolone,
http://linkedlifedata.com/resource/pubmed/chemical/Progesterone,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/diazepam-binding inhibitor receptor
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0014-2999
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
269
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
17-24
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7530205-1-Methyl-3-isobutylxanthine,
pubmed-meshheading:7530205-Adrenalectomy,
pubmed-meshheading:7530205-Animals,
pubmed-meshheading:7530205-Benzodiazepinones,
pubmed-meshheading:7530205-Bucladesine,
pubmed-meshheading:7530205-Cerebral Cortex,
pubmed-meshheading:7530205-Cyclic AMP,
pubmed-meshheading:7530205-Cycloheximide,
pubmed-meshheading:7530205-Dose-Response Relationship, Drug,
pubmed-meshheading:7530205-Forskolin,
pubmed-meshheading:7530205-Isoquinolines,
pubmed-meshheading:7530205-Male,
pubmed-meshheading:7530205-Orchiectomy,
pubmed-meshheading:7530205-Pregnenolone,
pubmed-meshheading:7530205-Progesterone,
pubmed-meshheading:7530205-Rats,
pubmed-meshheading:7530205-Rats, Sprague-Dawley,
pubmed-meshheading:7530205-Receptors, Cytoplasmic and Nuclear
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pubmed:year |
1994
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pubmed:articleTitle |
Modulatory mechanisms of cyclic AMP-stimulated steroid content in rat brain cortex.
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pubmed:affiliation |
Dip. to Medicina Sperimentale, Università di Roma, Tor Vergata, Italy.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, Non-U.S. Gov't
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