Linked Life Data

7529796

Source:http://linkedlifedata.com/resource/pubmed/id/7529796

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-2-16
pubmed:abstractText
Cytokines are believed to regulate Ig class switching, in part, through selective modulation of germ-line constant heavy (CH) gene transcription. B cell activators such as LPS or activated T cell membranes also influence germ-line CH RNA expression in the absence of exogenous cytokines. In this report we determined whether multivalent Ag receptor cross-linking, utilizing dextran-conjugated anti-IgD Abs (alpha delta-dex), could also regulate germ-line CH RNA expression. We demonstrated that alpha delta-dex markedly inhibited germ-line epsilon RNA expression, but strongly augmented germ-line gamma 1 RNA, in LPS + IL-4-stimulated cultures. This was correlated with > 90% alpha delta-dex-mediated suppression in the secretion of IgE and generation of membrane (m)IgE+ cells, and a more modest 50% reduction in IgG1 synthesis and mIgG1+ cells. Furthermore, alpha delta-dex inhibited the LPS induction of both gamma 3 and gamma 2b germ-line RNA and the associated secretion of IgG3 and IgG2b. A similar alpha delta-dex-mediated suppression of germ-line gamma 2a RNA and IgG2a secretion in LPS + IFN-gamma-stimulated cultures was observed. By contrast, activation of resting B cells with alpha delta-dex alone led to induction of germ-line gamma 3, gamma 1, and gamma 2b RNA but did not stimulate detectable expression of RNA specific for gamma 2a or epsilon. These studies demonstrate that: 1) germ-line gamma 1 gene expression is regulated uniquely, 2) germ-line transcription and switch recombination can be dissociated, 3) the germ-line transcription of each IgG isotype has an independent pattern of regulation, and 4) cross-linking of the Ag receptor, by itself, can stimulate small amounts of germ-line CH RNA.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
154
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1223-31
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:7529796-Animals, pubmed-meshheading:7529796-B-Lymphocytes, pubmed-meshheading:7529796-Blotting, Northern, pubmed-meshheading:7529796-Cells, Cultured, pubmed-meshheading:7529796-Dextrans, pubmed-meshheading:7529796-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:7529796-Female, pubmed-meshheading:7529796-Gene Expression Regulation, pubmed-meshheading:7529796-Gene Rearrangement, B-Lymphocyte, pubmed-meshheading:7529796-Immunoglobulin Class Switching, pubmed-meshheading:7529796-Immunoglobulin Constant Regions, pubmed-meshheading:7529796-Immunoglobulin D, pubmed-meshheading:7529796-Immunoglobulin Heavy Chains, pubmed-meshheading:7529796-Interferon-gamma, pubmed-meshheading:7529796-Interleukin-4, pubmed-meshheading:7529796-Lipopolysaccharides, pubmed-meshheading:7529796-Mice, pubmed-meshheading:7529796-Mice, Inbred DBA, pubmed-meshheading:7529796-RNA, Messenger, pubmed-meshheading:7529796-Single-Strand Specific DNA and RNA Endonucleases
pubmed:year
1995
pubmed:articleTitle
Antigen receptor cross-linking differentially regulates germ-line CH ribonucleic acid expression in murine B cells.
pubmed:affiliation
Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't