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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1995-2-10
|
| pubmed:abstractText |
We describe the construction of a plasmid carrying a DNA cassette encoding the influenza virus hemagglutinin (HA1) epitope. Insertion of this cassette into the coding region creates a gene encoding an epitope-tagged protein. The tagged protein can be detected and quantified by using a commercially available anti-HA1 antibody. A one-step procedure introduces the epitope at any restriction site within a gene while maintaining the open reading frame. An example of the application of this technique is presented and the different uses of the cassette are discussed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0736-6205
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
17
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
560-5
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7529515-Amino Acid Sequence,
pubmed-meshheading:7529515-Animals,
pubmed-meshheading:7529515-Base Sequence,
pubmed-meshheading:7529515-Drosophila melanogaster,
pubmed-meshheading:7529515-Epitopes,
pubmed-meshheading:7529515-Hemagglutinin Glycoproteins, Influenza Virus,
pubmed-meshheading:7529515-Hemagglutinins, Viral,
pubmed-meshheading:7529515-Molecular Sequence Data,
pubmed-meshheading:7529515-Plasmids
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Strategy for epitope tagging the protein-coding region of any gene.
|
| pubmed:affiliation |
Case Western Reserve University, School of Medicine, Cleveland, OH.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|