Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1995-2-9
|
| pubmed:abstractText |
Dendritic cells (DC) are potent APCs, able to induce efficiently primary T cell-mediated responses to foreign Ags. To assess the efficiency of DC, as compared with other APC types, in the in vivo presentation of self-Ags to CD4+ T cells, we analyzed processing and presentation to class II-restricted T cells of endogenous naturally processed self-epitopes constitutively expressed by mouse APC. Mouse beta 2-microglobulin (m beta 2-m) peptides corresponding to residues 26-39 and 24-36 are constitutively presented, in mice expressing m beta 2-m, by I-Ad and I-Ed molecules respectively, as demonstrated by activation of m beta 2-m-specific T cell hybridomas generated in BALB/c beta 2-m-deficient mice. These dominant, naturally processed self-epitopes of m beta 2-m are presented by APC from a variety of tissues, including the thymus. To analyze the relative efficiency of different APC populations in the presentation of self-beta 2-m, the ability of purified DC, macrophages, and large or small B cells to stimulate m beta 2-m-specific T cell hybridomas was tested. Naturally processed self-m beta 2-m epitopes are constitutively presented to T cells by any class II-positive APC tested, but with highest efficiency by splenic and thymic DC, followed by macrophages, large B cells, and small B cells. This hierarchy of self-beta 2-m presentation does not depend on differential processing capacity of these APC populations, and it correlates with expression of CTLA-4 ligands and ICAM-1 molecules, rather than with expression of class II molecules.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
154
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
536-44
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7529278-Amino Acid Sequence,
pubmed-meshheading:7529278-Animals,
pubmed-meshheading:7529278-Antigen Presentation,
pubmed-meshheading:7529278-Antigen-Presenting Cells,
pubmed-meshheading:7529278-Autoantigens,
pubmed-meshheading:7529278-B-Lymphocytes,
pubmed-meshheading:7529278-CD4-Positive T-Lymphocytes,
pubmed-meshheading:7529278-Dendritic Cells,
pubmed-meshheading:7529278-Epitopes,
pubmed-meshheading:7529278-Flow Cytometry,
pubmed-meshheading:7529278-Histocompatibility Antigens Class II,
pubmed-meshheading:7529278-Hybridomas,
pubmed-meshheading:7529278-Macrophages,
pubmed-meshheading:7529278-Mice,
pubmed-meshheading:7529278-Mice, Inbred BALB C,
pubmed-meshheading:7529278-Molecular Sequence Data,
pubmed-meshheading:7529278-Thymus Gland,
pubmed-meshheading:7529278-beta 2-Microglobulin
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Dendritic cells are the most efficient in presenting endogenous naturally processed self-epitopes to class II-restricted T cells.
|
| pubmed:affiliation |
Roche Milano Richerche, Italy.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|