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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1995-2-3
|
| pubmed:abstractText |
A hormone-dependent, clonal carcinoma cell line, designated RM22-F5, was derived from a malignant mammary mixed tumor spontaneously arising in an outbred old female Wistar rat. These cells expressed keratin and desmosomal protein and formed epithelial monolayers in a growth factor and hormone-supplemented medium (LHC-8) containing 10% fetal bovine serum (E-type cells). Cells subcultured for 6 to 8 passages in RPMI 1640 medium containing 10% fetal bovine serum without supplements appeared to be fibroblastic and expressed vimentin (F-type cells). The shift to a fibroblast-like morphology was associated with a more malignant phenotype which included rapid, hormone-independent growth and invasive sarcoma-like character in nude mice. F-type cells were no longer able to express their original epithelial phenotype in LHC-8 medium. Cytogenetic analysis revealed that both E- and F-type cells had essentially the same karyotype. Analysis of PCR-amplified DNA further demonstrated a point mutation of the H-ras-1 gene at codon 12 and loss of the normal H-ras-1 allele in both cell types. Genetic tagging of E-type cells with the neomycin-resistance gene resulted in the generation of F-type cells with neomycin resistance in RPMI 1640 medium, suggesting that F-type cells are a malignant variant of E-type cells arising during in vitro culture. Somatic cell fusion between E- and F-type cells revealed that with most hybrid clones tested, the fibroblast-like phenotype was greatly suppressed. These results suggest that an irreversible phenotypic transition, representative of tumor progression from hormone-dependent adenocarcinoma to more malignant hormone-independent spindle carcinoma cells, is a recessive event and may involve loss of a suppressor function.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0008-5472
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
55
|
| pubmed:geneSymbol |
H-ras-1
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
399-407
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7529136-Adenocarcinoma,
pubmed-meshheading:7529136-Animals,
pubmed-meshheading:7529136-Base Sequence,
pubmed-meshheading:7529136-Carcinoma,
pubmed-meshheading:7529136-Cell Division,
pubmed-meshheading:7529136-Cell Fusion,
pubmed-meshheading:7529136-Cell Transformation, Neoplastic,
pubmed-meshheading:7529136-Culture Media,
pubmed-meshheading:7529136-Desmosomes,
pubmed-meshheading:7529136-Female,
pubmed-meshheading:7529136-Flow Cytometry,
pubmed-meshheading:7529136-Genes, ras,
pubmed-meshheading:7529136-Keratins,
pubmed-meshheading:7529136-Mammary Neoplasms, Animal,
pubmed-meshheading:7529136-Mice,
pubmed-meshheading:7529136-Mice, Nude,
pubmed-meshheading:7529136-Molecular Sequence Data,
pubmed-meshheading:7529136-Neomycin,
pubmed-meshheading:7529136-Neoplasm Invasiveness,
pubmed-meshheading:7529136-Neoplasms, Hormone-Dependent,
pubmed-meshheading:7529136-Phenotype,
pubmed-meshheading:7529136-Rats,
pubmed-meshheading:7529136-Rats, Wistar,
pubmed-meshheading:7529136-Transfection,
pubmed-meshheading:7529136-Tumor Cells, Cultured,
pubmed-meshheading:7529136-Vimentin
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Progression of hormone-dependent adenocarcinoma cells to hormone-independent spindle carcinoma cells in vitro in a clonal spontaneous rat mammary tumor cell line.
|
| pubmed:affiliation |
Laboratory of Biological Chemistry, National Institute on Aging, Baltimore, Maryland 21224.
|
| pubmed:publicationType |
Journal Article
|