Linked Life Data

7529060

Source:http://linkedlifedata.com/resource/pubmed/id/7529060

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-2-3
pubmed:abstractText
The number of CD34+ cells in the peripheral blood of cancer patients is known to be increased following the administration of high dose chemotherapy and hematopoietic growth factors. These so-called peripheral blood stem cell grafts are now frequently used for autologous transplantation of patients with malignancies. In this report, we address the question of whether true long-term repopulating pluripotent hematopoietic stem cells (PHSC) are mobilized into peripheral blood following chemotherapy plus granulocyte/macrophage colony-stimulating factor (GM-CSF) or granulocyte colony-stimulating factor (G-CSF) mobilization. We have examined the presence of stem cells in mobilized peripheral blood (MPB) by using an antibody to the human Thy-1 molecule to stain the CD34+Lineage- (Lin-) population. The kinetics of mobilization of CD34+Thy-1+ Lin- cells into peripheral blood were studied, and the percentage of cells with this phenotype was found to vary widely depending on the day of leukapheresis. A CD34+Thy-1+Lin- cell population, potentially containing PHSCs, was isolated by fluorescence activated cell sorting (FACS) and analyzed for activity. The multilineage differentiative capacity of this candidate stem cell-containing population in MPB was determined using an in vitro long-term culture system, in which cobblestone area formation was used as a means of detecting PHSCs. We also measured repopulating capacity by using two in vivo models in which severe combined immunodeficiency (SCID)-hu mice were implanted with human fetal bone or thymus grafts. Using these assays, we show that the highest frequency of cobblestone area-forming cells (CAFC) after 7 weeks of culture was observed in a subpopulation of CD34+Lin- cells, which expressed low levels of Thy-1. This cell population was capable of producing both B and myeloid cells, and maintaining CD34+Lin- cells in these long term cultures. Moreover, the CD34+Thy-1+Lin- cell subset possessed a higher ability to engraft and to demonstrate multilineage differentiative potential at 8 weeks in the SCID-hu bone assay. However, in the SCID-hu thymus model, both Thy-1+ and Thy-1- subpopulations were capable of donor T-cell engraftment at 6 weeks, suggesting the presence of cells capable of initiating T lymphopoiesis in both populations.(ABSTRACT TRUNCATED AT 400 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
368-78
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7529060-Animals, pubmed-meshheading:7529060-Antigens, CD, pubmed-meshheading:7529060-Antigens, CD34, pubmed-meshheading:7529060-Antigens, Thy-1, pubmed-meshheading:7529060-Blood Cell Count, pubmed-meshheading:7529060-Bone Marrow, pubmed-meshheading:7529060-Chimera, pubmed-meshheading:7529060-Cyclophosphamide, pubmed-meshheading:7529060-Etoposide, pubmed-meshheading:7529060-Flow Cytometry, pubmed-meshheading:7529060-Graft Survival, pubmed-meshheading:7529060-Granulocyte Colony-Stimulating Factor, pubmed-meshheading:7529060-Hematopoiesis, pubmed-meshheading:7529060-Hematopoietic Stem Cell Transplantation, pubmed-meshheading:7529060-Hematopoietic Stem Cells, pubmed-meshheading:7529060-Humans, pubmed-meshheading:7529060-Mice, pubmed-meshheading:7529060-Mice, SCID, pubmed-meshheading:7529060-Neoplasms, pubmed-meshheading:7529060-Time Factors, pubmed-meshheading:7529060-Transplantation, Heterologous
pubmed:year
1995
pubmed:articleTitle
Enrichment of human hematopoietic stem cell activity in the CD34+Thy-1+Lin- subpopulation from mobilized peripheral blood.
pubmed:affiliation
SyStemix Inc., Palo Alto, CA 94304.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.