Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1995-1-30
pubmed:abstractText
Several accessory molecules were shown to play important roles in T cell functions and be in close proximity to the T cell receptor (TcR/CD3). The L-selectin molecule (Leu8, LAM1-1, LECAM1) also plays an important role in lymphocyte homing and proliferation. We were interested in determining the proximity of this molecule to the TcR/CD3 complex on live peripheral human T cells. Using a fluorescence energy transfer method, designed to study individual cells, we could show that L-selectin is within 170 A of the TcR/CD3 complex. Monoclonal antibody directed against the LAM1-1 (Leu8) epitope of the L-selectin molecule suppressed the mitogenic activity of antibodies specific for various CD3 epitopes in vitro. Intracellular Ca2+ mobilization obtained with wt31 followed by cross-linking antibody or with anti-CD3 was not influenced by anti-Leu8 antibody. Also antibody directed against the LAM1-1 epitope did not influence the binding of the mitogenic antibodies, as shown by fluorescence-based flow cytometry. Therefore, we suggest that binding of TcR/CD3 bound mitogenic antibodies to accessory cell Fc receptors may be hindered by antibodies bound to the close proximity L-selectin molecules.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0818-9641
pubmed:author
pubmed:issnType
Print
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
319-25
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed-meshheading:7528722-Antigens, CD3, pubmed-meshheading:7528722-Calcium, pubmed-meshheading:7528722-Cell Adhesion Molecules, pubmed-meshheading:7528722-Cell Division, pubmed-meshheading:7528722-DNA Replication, pubmed-meshheading:7528722-Energy Transfer, pubmed-meshheading:7528722-Epitopes, pubmed-meshheading:7528722-Humans, pubmed-meshheading:7528722-L-Selectin, pubmed-meshheading:7528722-Lasers, pubmed-meshheading:7528722-Lymphocyte Activation, pubmed-meshheading:7528722-Muromonab-CD3, pubmed-meshheading:7528722-Photochemistry, pubmed-meshheading:7528722-Protein Binding, pubmed-meshheading:7528722-Receptor-CD3 Complex, Antigen, T-Cell, pubmed-meshheading:7528722-Receptors, Fc, pubmed-meshheading:7528722-Signal Transduction, pubmed-meshheading:7528722-Spectrometry, Fluorescence, pubmed-meshheading:7528722-T-Lymphocytes
pubmed:year
1994
pubmed:articleTitle
The L-selectin (Leu8) molecule is associated with the TcR/CD3 receptor; fluorescence energy transfer measurements on live cells.
pubmed:affiliation
Department of Biophysics, University Medical School of Debrecen, Hungary.
pubmed:publicationType
Journal Article