rdf:type |
|
lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0014274,
umls-concept:C0016315,
umls-concept:C0108779,
umls-concept:C0125090,
umls-concept:C0242485,
umls-concept:C0332281,
umls-concept:C0567416,
umls-concept:C0597357,
umls-concept:C1335808,
umls-concept:C1548795
|
pubmed:issue |
4
|
pubmed:dateCreated |
1995-1-30
|
pubmed:abstractText |
Several accessory molecules were shown to play important roles in T cell functions and be in close proximity to the T cell receptor (TcR/CD3). The L-selectin molecule (Leu8, LAM1-1, LECAM1) also plays an important role in lymphocyte homing and proliferation. We were interested in determining the proximity of this molecule to the TcR/CD3 complex on live peripheral human T cells. Using a fluorescence energy transfer method, designed to study individual cells, we could show that L-selectin is within 170 A of the TcR/CD3 complex. Monoclonal antibody directed against the LAM1-1 (Leu8) epitope of the L-selectin molecule suppressed the mitogenic activity of antibodies specific for various CD3 epitopes in vitro. Intracellular Ca2+ mobilization obtained with wt31 followed by cross-linking antibody or with anti-CD3 was not influenced by anti-Leu8 antibody. Also antibody directed against the LAM1-1 epitope did not influence the binding of the mitogenic antibodies, as shown by fluorescence-based flow cytometry. Therefore, we suggest that binding of TcR/CD3 bound mitogenic antibodies to accessory cell Fc receptors may be hindered by antibodies bound to the close proximity L-selectin molecules.
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pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/L-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Muromonab-CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor-CD3 Complex, Antigen...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Fc
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
|
pubmed:issn |
0818-9641
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pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
72
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
319-25
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:7528722-Antigens, CD3,
pubmed-meshheading:7528722-Calcium,
pubmed-meshheading:7528722-Cell Adhesion Molecules,
pubmed-meshheading:7528722-Cell Division,
pubmed-meshheading:7528722-DNA Replication,
pubmed-meshheading:7528722-Energy Transfer,
pubmed-meshheading:7528722-Epitopes,
pubmed-meshheading:7528722-Humans,
pubmed-meshheading:7528722-L-Selectin,
pubmed-meshheading:7528722-Lasers,
pubmed-meshheading:7528722-Lymphocyte Activation,
pubmed-meshheading:7528722-Muromonab-CD3,
pubmed-meshheading:7528722-Photochemistry,
pubmed-meshheading:7528722-Protein Binding,
pubmed-meshheading:7528722-Receptor-CD3 Complex, Antigen, T-Cell,
pubmed-meshheading:7528722-Receptors, Fc,
pubmed-meshheading:7528722-Signal Transduction,
pubmed-meshheading:7528722-Spectrometry, Fluorescence,
pubmed-meshheading:7528722-T-Lymphocytes
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pubmed:year |
1994
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pubmed:articleTitle |
The L-selectin (Leu8) molecule is associated with the TcR/CD3 receptor; fluorescence energy transfer measurements on live cells.
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pubmed:affiliation |
Department of Biophysics, University Medical School of Debrecen, Hungary.
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pubmed:publicationType |
Journal Article
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