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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
25
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pubmed:dateCreated |
1995-1-11
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pubmed:abstractText |
To study the role of B-cell antigen CD40 in immune responses, mouse embryonic stem (ES) cells in which both copies of the gene encoding CD40 had been disrupted by homologous recombination were injected in RAG-2 (recombination-activating gene-2)-deficient blastocysts to generate chimeras in which all mature lymphocytes are derived from the CD40-deficient ES cells. T- and B-cell number and phenotype were normal in the CD40-/- chimeras. However, B cells failed to proliferate and undergo isotype switching in vitro in response to soluble CD40 ligand (sCD40L) with interleukin 4 (IL-4) but responded normally to lipopolysaccharide (LPS) with IL-4. CD40-/- chimeras completely failed to mount an antigen-specific antibody response or to develop germinal centers following immunization with the T cell-dependent (TD) antigen keyhole limpet hemocyanin. In contrast, CD40-/- mutant mice responded normally to the T cell-independent (TI) antigens, 2,4,6-trinitrophenyl (TNP)-LPS and TNP-Ficoll. The most noticeable alteration in the serum immunoglobulin levels of young (6-8 weeks old) CD40-/- animals was absence of IgE and severe decrease of IgG1 and IgG2a. These results confirm the essential role of CD40- CD40L interactions in the antibody response to TD antigens and in isotype switching.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-1374165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-1378865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-1547487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-1554497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-1701824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-1706382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-2145580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-2456366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-2475341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-6609363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-7534202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-7678782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-7679206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-7679801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-7681469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-7681587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-7688031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-7693850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-7908915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-8011289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-8094231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-8104709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7527552-8506294
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Rag2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/V(D)J recombination activating...
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
12135-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7527552-Animals,
pubmed-meshheading:7527552-Antibody Formation,
pubmed-meshheading:7527552-Antigens, CD,
pubmed-meshheading:7527552-Antigens, CD40,
pubmed-meshheading:7527552-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:7527552-B-Lymphocytes,
pubmed-meshheading:7527552-Blastocyst,
pubmed-meshheading:7527552-DNA-Binding Proteins,
pubmed-meshheading:7527552-Flow Cytometry,
pubmed-meshheading:7527552-Genetic Complementation Test,
pubmed-meshheading:7527552-Genomic Library,
pubmed-meshheading:7527552-Immunoglobulin E,
pubmed-meshheading:7527552-Immunoglobulin G,
pubmed-meshheading:7527552-Immunoglobulin M,
pubmed-meshheading:7527552-Lymphocyte Activation,
pubmed-meshheading:7527552-Lymphocytes,
pubmed-meshheading:7527552-Mice,
pubmed-meshheading:7527552-Peyer's Patches,
pubmed-meshheading:7527552-Proteins,
pubmed-meshheading:7527552-Recombination, Genetic,
pubmed-meshheading:7527552-Reference Values,
pubmed-meshheading:7527552-Restriction Mapping,
pubmed-meshheading:7527552-T-Lymphocytes
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pubmed:year |
1994
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pubmed:articleTitle |
CD40-deficient mice generated by recombination-activating gene-2-deficient blastocyst complementation.
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pubmed:affiliation |
Division of Immunology, Children's Hospital, Boston, MA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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