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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1995-1-12
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pubmed:abstractText |
Endothelins (ETs) are important regulators of growth and function in many endocrine tissues. This study was designed to verify the expression of ETs in a series of normal human pituitaries and pituitary adenomas. We examined 13 normal pituitaries and 58 pituitary adenomas for the presence of immunoreactive (ir) ET-1 and ET-3. No ir-ET-1 was detected in any of the 13 normal pituitaries, whereas ir-ET-3 was observed in 4 of 13 (31%) cases. In contrast, 48% (28 of 58) of pituitary adenomas display immunoreactivity for ET-1, whereas 31% (18 of 58) show immunoreactivity for ET-3. With respect to the type of tumors, staining was as follows: nonfunctioning adenomas: ET-1, 14 of 33; ET-3, 9 of 33; somatotropinomas: ET-1, 8 of 16; ET-3, 6 of 16; corticotropinomas: ET-1, 5 of 5; ET-3, 2 of 5; and prolactinomas; ET-1, 1 of 4; ET-3, 1 of 4. Using double immunostaining, we found the colocalization of ET-3 in normal pituitaries and of ET-1 and ET-3 in pituitaries adenomas in each hormone-secreting cell. In Cushing adenomas, ET-1 was coexpressed in corticotropic cells in all 5 cases (100%). In the same tumors, by reverse transcriptase polymerase chain reaction, we investigated the presence of ET-1 and ET-3 messenger ribonucleic acids and found that they are expressed, respectively, in 18 of 21 and 7 of 11 tumors examined. Our findings demonstrate that pituitary adenomas frequently display ET-1 as well as ET-3 immunoreactivity, in contrast to normal pituitaries, in which only ET-3 was found.(ABSTRACT TRUNCATED AT 250 WORDS)
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0021-972X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1864-70
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7527415-Adenoma,
pubmed-meshheading:7527415-Aged,
pubmed-meshheading:7527415-Aged, 80 and over,
pubmed-meshheading:7527415-Base Sequence,
pubmed-meshheading:7527415-Endothelins,
pubmed-meshheading:7527415-Female,
pubmed-meshheading:7527415-Gene Expression,
pubmed-meshheading:7527415-Humans,
pubmed-meshheading:7527415-Immunohistochemistry,
pubmed-meshheading:7527415-Male,
pubmed-meshheading:7527415-Middle Aged,
pubmed-meshheading:7527415-Molecular Sequence Data,
pubmed-meshheading:7527415-Pituitary Gland, Anterior,
pubmed-meshheading:7527415-Pituitary Neoplasms,
pubmed-meshheading:7527415-Polymerase Chain Reaction,
pubmed-meshheading:7527415-RNA, Messenger,
pubmed-meshheading:7527415-RNA-Directed DNA Polymerase
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pubmed:year |
1994
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pubmed:articleTitle |
Endothelin expression in normal human anterior pituitaries and pituitary adenomas.
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pubmed:affiliation |
Department of Neurosurgery, Hospital of Mannheim, University of Heidelberg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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